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Showing: 1021 - 1040 of 1286 results

  4. pZE21/UBP1/ClpS

    Plasmid
    #98566
    Purpose
    Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon
    Depositor
    George Church
    Article
    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11
    Inserts
    Truncated ubiquitin cleavase, codon optimized
    ATP-dependent Clp protease adapter protein
    Expression
    Bacterial
    Mutation
    In a synthetic operon downstream of UBP1 and Remo…
    Promoter
    Same as UBP1 (operon) and Tet promoter (aTc induc…
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98566-plasmid-map-sequence-id-203067

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  8. PWPXLD-GFP-Numb-S276D

    Plasmid
    #133752
    Purpose
    Numb with mutation at serine 276 converted to aspartic acid cand tagged with GFP cloned into PWPXLD
    Depositor
    Chay Kuo
    Article
    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4
    Insert
    NUMB endocytic adaptor protein (Numb Mouse)
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    serine 276 converted to aspartic acid
    Promoter
    EF1-alpha
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    133752-plasmid-map-sequence-id-264923

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  13. PWPXLD-EGFR-P667A

    Plasmid
    #133750
    Purpose
    EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid
    Depositor
    Chay Kuo
    Article
    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4
    Insert
    Mutant P667A EGFR-HA (EGFR Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    proline 667 converted to alanine (Please see depo…
    Promoter
    EF1-alpha
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    133750-plasmid-map-sequence-id-264885

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  17. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

    Plasmid
    #196196
    Purpose
    homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus
    Depositor
    Jürgen Knoblich
    Article
    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75
    Insert
    G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
    Use
    Homology repair donor
    Tags
    V5-APEX2-mScarlet-I
    Available Since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    196196-plasmid-map-sequence-id-385065

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