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pRB30

Plasmid

#165480

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-FLAG-10x His

Expression

Mammalian

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-665 (attR4-attR2)

Plasmid

#161885

Purpose

Multisite Gateway (attR4-attR2) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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Auxiliary Δ

Plasmid

#153355

Purpose

Provides End-to-End linker 4Δ for level 2 cloning

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

End-to-End linker 4Δ

Use

Synthetic Biology

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary Γ

Plasmid

#153354

Purpose

Provides End-to-End linker 4Γ for level 2 cloning

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

End-to-End linker 4Γ

Use

Synthetic Biology

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary B

Plasmid

#153353

Purpose

Provides End-to-End linker 4B for level 2 cloning

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

End-to-End linker 4B

Use

Synthetic Biology

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary 3

Plasmid

#153351

Purpose

Provides Middle-to-End linker 3 for level 2 cloning

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

Middle-to-End linker 3

Use

Synthetic Biology

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary 1

Plasmid

#153349

Purpose

Provides Middle-to-End linker 1 for level 2 cloning

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

Middle-to-End linker 1

Use

Synthetic Biology

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pTK746

Plasmid

#114712

Purpose

Express NuMA(1-2115ΔNLS)5A-3-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-2115ΔNLS)5A-3-RFP-Nano

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTK713

Plasmid

#114699

Purpose

Express NuMA(1-2115ΔNLS)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-2115ΔNLS)-RFP-Nano

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLK_pFa

Plasmid

#114229

Purpose

POC12367, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLK_dFq

Plasmid

#114237

Purpose

POC12377, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type d-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLP_pFa

Plasmid

#114239

Purpose

POC12379, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pTU1-D-recJD

Plasmid

#112784

Purpose

Downstream homology arm, RecJ is a ssDNA specific exonuclease, involved in genome repair and maintenance

Depositor

Chris French

Article

Wicke et al bioRxiv 185108

Insert

recJD

Expression

Bacterial

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pWT046b

Plasmid

#107898

Purpose

W2.3 plasmid. Contains PTetO-SD8-BE2, PRha-sgRNA4 and is part of CAMERA 2.3

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PRha-sgRNA4

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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LIIc F 1-2 RNAi

Plasmid

#54402

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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LII dy 1-3

Plasmid

#54374

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

dummy 1-3

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LII dy 4-5

Plasmid

#54376

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

dummy 4-5

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LII dy 2-3

Plasmid

#54375

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

dummy 2-3

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pFUS_B7

Plasmid

#31024

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pFUS_B8

Plasmid

#31025

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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