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pCMU-MITr

Plasmid

#61198

Purpose

Fluorescent reporter for mitochondria ScCOX4sp-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

ScCOX4sp-mCherry

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-GFP

Plasmid

#112851

Purpose

General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-GFP

Use

Synthetic Biology

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_N_VPS26C

Plasmid

#209689

Purpose

Expresses MAC-tagged VPS26C in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS26C (VPS26C Human)

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCLX-pTF-R1-DEST-R2-EBR65

Plasmid

#45952

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCZGY3196

Plasmid

#172696

Purpose

Prgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

Prgef-1

Tags

GFP 1-10

Expression

Worm

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-LUC (w158-1)

Plasmid

#17473

Purpose

Gateway entry vector, Firefly Luciferase

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Entry vector

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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p16 nlsGPS1-NR

Plasmid

#101082

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

nlsGPS1-NR

Tags

cMyc

Expression

Plant

Mutation

AtGAI E51K E54R, AtGID1C S114A F115A

Promoter

p16 (AT3G60245)

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_TIAL1

Plasmid

#106090

Purpose

gene expression of FlagHA-TIAL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TIAL1 (TIAL1 Human)

Tags

FlagHA

Expression

Mammalian

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ462

Plasmid

#187970

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMU-ERr

Plasmid

#61170

Purpose

Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtWAK2-mCherry-HDEL

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

April 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCMU-PDESr

Plasmid

#61206

Purpose

Fluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPDLP1

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMOS031: superecliptic pHluorin (endoplasmic reticulum)

Plasmid

#163051

Purpose

superecliptic pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCMU-GAr

Plasmid

#61172

Purpose

Fluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

GmMAN49-mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pME tdTomato-CAAX

Plasmid

#135204

Purpose

Red fluorescent tag

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

tdTomato

Use

Synthetic Biology

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ141D

Plasmid

#69293

Purpose

Gateway entry vector; single gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pDR-GW AT1.03YEMK

Plasmid

#28004

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03YEMK

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

M60Y, G131E, K132M, 133K

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pLentiN 16E6no*

Plasmid

#37445

Purpose

Lentiviral expression of HPV 16E6no* in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 16E6no* (E6 HPV)

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

V42L

Promoter

CMV

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
MAC_C_ROS1

Plasmid

#187766

Purpose

MAC-tagged gene expression of human ROS1

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

ROS1 (ROS1 Human)

Expression

Mammalian

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-2a-tdTomato

Plasmid

#67707

Purpose

3' entry 2A-tdTomato

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

2A-tdTomato

Mutation

G172S (please see depositor's comments below)

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pZmUBQ:GW

Plasmid

#132358

Purpose

Expression of CDS from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Article

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Type

Empty backbone

Tags

YFP

Expression

Plant

Promoter

pZmUBQ

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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