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Ai140 (TIT2L-GFP-ICL-tTA2) targeting vector

Plasmid

#114427

Purpose

Target a Cre-dependent GFP and a tTA2 expression cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GFP, tTA2

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Promoter

PGK, CAG

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-NLS-actin

Plasmid

#118380

Purpose

Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

NLS

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)

Plasmid

#110572

Purpose

Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD-stRBS-aacC1

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB-SPRTNwt-FL

Plasmid

#110217

Purpose

Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Expression

Bacterial

Mutation

P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAH1 (lenti hLPL-FLAG)

Plasmid

#102448

Purpose

FLAG-tagged human LPL in lentiviral vector

Depositor

Brandon Davies

Article

Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

Insert

Lipoprotein lipase (LPL Human)

Use

Lentiviral

Tags

6x His and FLAG

Promoter

CMV

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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cacnb1b (rat) in pMT2 vector

Plasmid

#107423

Purpose

Expresses Calcium channel beta1b auxillary subunit in mammalian cells.

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 1997 Feb 15;17(4):1330-8.

Insert

calcium channel beta1b auxillary subunit

Expression

Mammalian

Promoter

Ad MLP

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-iC++

Plasmid

#98165

Purpose

artificial anion conducting channelrhodopsin (aACR). Activation max 488 nm; off-kinetics 35 ms. Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct iC++ gene

Tags

mCherry

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, V242R, T24…

Promoter

CMV (+enhancer)

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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Ai95(RCL-GCaMP6f) targeting vector

Plasmid

#61579

Purpose

Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

CAG-LSL-GCaMP6-fast

Use

Cre/Lox and Mouse Targeting

Promoter

CAG

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE GFP-Ago2 S387A

Plasmid

#60654

Purpose

GFP-Ago2 S387A mutant lentiviral vector

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Use

Lentiviral

Tags

GFP

Mutation

S387A

Promoter

EF1a

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-LplA(AILR,36)-NRX3b

Plasmid

#43919

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

AILR-LplA36-NRX3b (lplA Synthetic, Human)

Tags

2XHA

Expression

Mammalian

Promoter

Chicken beta actin

Available Since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-PES1

Plasmid

#38085

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

PES1 pescadillo ribosomal biogenesis factor 1 (PES1 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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WZLneo-PPARγ2

Plasmid

#34566

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

PPARγ2 (Pparg Mouse)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

5'LTR

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only
pEBB-II-I-GFP beta isoform

Plasmid

#22147

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pGEX Smad3 C

Plasmid

#12634

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad3 C (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

C segment of Smad3 (aa 199-425)

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
tetO-ESRRA

Plasmid

#170683

Purpose

doxycycline-inducible overexpression of ESRRA

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

ESRRA (ESRRA Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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C1-MPAct(delta1-6)-mRuby3

Plasmid

#155223

Purpose

F-actin bidning mutant of MPAct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Mutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b

Tags

mRuby3

Expression

Mammalian

Mutation

Deletion of first 6 AA from F-tractin (9-14 from …

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Mouse GFP-PRMT7 Wild Type

Plasmid

#65056

Purpose

Expresses wild type mouse GFP-PRMT7

Depositor

Steven Clarke

Article

Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

Insert

PRMT7 (Prmt7 Mouse)

Use

Bacterial propagation

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P3nmt1

Plasmid

#39280

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus

Available Since

Aug. 14, 2012

Availability

Academic Institutions and Nonprofits only

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NG-ABE-NW

Plasmid

#240622

Purpose

Adenine base editor with narrowed editing window, recognizing NG PAM

Depositor

Tingting Jiang

Article

Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

Insert

TadA8e(N108Q, M151C, Q154Y)-linker-nCas9 (D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)

Use

CRISPR

Expression

Mammalian

Mutation

TadA8e(N108Q, M151C, Q154Y), Cas9 (D10A, L1111R, …

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pEM047 [T7-assisted CROPseq sgRNA expression vector]

Plasmid

#224899

Purpose

Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA

Depositor

Thomas Norman

Article

Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi

Inserts

eGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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