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pAP-U2-1

Plasmid

#236472

Purpose

Plasmid for integration at the native URA3 locus with 3xmCherry and 3xGFP under control of the strong bidirectional SpH2A/B promoter.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

spH2Ap-3xmCherry spH2Bp-3xGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-rtTA

Plasmid

#233202

Purpose

Retroviral expression of rtTA for dox-inducible activation from a TRE promoter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

rtTA

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mRuby2

Plasmid

#233155

Purpose

Retroviral expression of fluorescent mRuby2 reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mRuby2

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pRC065d

Plasmid

#225979

Purpose

dRxCas13d expressed from J23107 promoter with J23119-driven crRNA (non-cognate DR)

Depositor

Jesse Zalatan

Article

Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

Inserts

dRxCas13d
buj1 crRNA (non-cognate DR) targets the unstructured RBS

Use

CRISPR

Promoter

J23107 and J23119

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-EGFPCAAX

Plasmid

#232140

Purpose

Expresses EGFP with a CAAX motif under control of a CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

EGFPCAAX

Use

Zebrafish expression

Promoter

CMV

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ mogat3b-FLAG

Plasmid

#232145

Purpose

Expresses zebrafish mogat3b with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat3b

Use

Zebrafish expression

Tags

FLAG

Promoter

CMV

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHW622

Plasmid

#229868

Purpose

Entry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)

Depositor

Han Wang

Article

Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

Insert

reverse FRT3 (rFRT3)

Expression

Worm

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCRII-mogat3b in situ probe

Plasmid

#232136

Purpose

zebrafish mogat3b anti-sense in situ probe, linearize with BamHI, T7 polymerase

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat3b

Use

In vitro transcription

Promoter

SP6/T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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AA516

Plasmid

#231118

Purpose

Fragmid fragment: (2A Selection) CBE activity-based selection intron

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

PuroN'-intron-PuroC'

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA509

Plasmid

#231116

Purpose

Fragmid fragment: (2A Selection) ABE activity-based selection intron

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

PuroN'-intron-PuroC'

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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UNK_ZnF1-3_pGEX

Plasmid

#219664

Purpose

Recombinant expression of UNK ZnF1-3 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al Nat Commun. 2024 Sep 27;15(1):8400. doi:

Insert

Unkempt Zinc Fingers 1-3

Tags

GST and SBP

Expression

Bacterial

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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UNK_ZnF4-6_pGEX

Plasmid

#219665

Purpose

Recombinant expression of UNK ZnF4-6 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al Nat Commun. 2024 Sep 27;15(1):8400. doi:

Insert

Unkempt Zinc Fingers 4-6

Tags

GST and SBP

Expression

Bacterial

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAM198

Plasmid

#227657

Purpose

pRS305 His-HRV3C-Rpn5 WT

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Tags

His-HRV3C-

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM200

Plasmid

#227659

Purpose

pRS305 3XFLAG-HRV3C-Rpn5 WT

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Tags

3X FLAG-HRV3C-

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM202

Plasmid

#227661

Purpose

pRS316 Rpn6 promoter-Rpn5 ORF-Rpn5 terminator

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-C9BoNT-A

Plasmid

#202490

Purpose

Expresses the nanobody C9 BoNT/A with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody C9 BoNT/A

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-Amp-T7-GFPuv

Plasmid

#202463

Purpose

Expresses GFPuv with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

GFPuv

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAM211 His-Ubp6

Plasmid

#226359

Purpose

Expression of yeast Ubp6

Depositor

Andreas Martin

Article

Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

Insert

ubp6 (UBP6 Budding Yeast)

Tags

His

Expression

Bacterial

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSCD-GSDMA-C

Plasmid

#226235

Purpose

Express GSDMA-C domain

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

Gasdermin A (Gsdma3 Mouse)

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSCD-cTEVP-RBD

Plasmid

#226233

Purpose

Express cTEVP-RBD fusion

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

cTEVP-RBD (TEVgp1 Tobacco etch virus)

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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