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pENTR FOXP3 WRR

Plasmid

#16365

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Expression

Bacterial

Mutation

WRR: N361W, E399R, E401R

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pENTR FOXP3 EARR

Plasmid

#16367

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Expression

Bacterial

Mutation

EARR: N361E, H365A, E399R, E401R

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pIF1-S39E-GFP

Plasmid

#206937

Purpose

Plasmid expressing the mutant S39E of human IF1 fused in C-terminal with GFP

Depositor

José Cuezva

Article

Romero-Carraminana et al Commun Biol. 2023 Aug 12;6(1):836. doi:

Insert

S39E mutant of human IF1 (ATPase inhibitory factor 1)

Tags

GFP

Expression

Mammalian

Mutation

Changed serine 39 for a glutamic acid

Promoter

CMV

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-DNMT3A

Plasmid

#220237

Purpose

Expresses dCAS9-DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-DNMT3A (dCas9-3A)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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E. coli DNA Polymerase III subunit Delta' (holB) in pet28PP

Plasmid

#217410

Purpose

Expresses E. coli DNA Polymerase III subunit delta' (holb) with a His-tag in bacterial cells

Depositor

Brian Kelch

Article

Landeck et al J Biol Chem. 2024 Apr;300(4):107166. doi

Insert

DNA polymerase III subunit Delta' (holB E. coli)

Tags

His

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBOB-Adcy3-HA

Plasmid

#245988

Purpose

Express HA C-terminal tagged wild-type mouse ADCY3 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pBOB-Adcy3-L278H-3xFlag

Plasmid

#245990

Purpose

Express 3xFlag C-terminal tagged mouse ADCY3-L278H mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

L278H

Promoter

CMV

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-3xFLAG-puro

Plasmid

#248155

Purpose

Lentiviral vector expressing human FCRL3 with 3xFLAG tags

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Tags

3x FLAG

Mutation

A 3xFLAG epitope tag is fused in frame to the C-t…

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(mem).iGlucoSnFR2

Plasmid

#244096

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).cpSFGFP.HaloTag

Plasmid

#244109

Purpose

Cytosolic expression of non-responsive circularly permuted Super Folder GFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

cpSFGFP

Use

AAV

Tags

HaloTag

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr201 RNF146 deltaWWE

Plasmid

#240331

Purpose

Entry vector for Gateway with RNF146 (WWE domain deletion)

Depositor

Junjie Chen

Article

Nie et al Mol Cell Proteomics. 2020 Dec;19(12):201

Insert

RNF146 (RNF146 Human)

Expression

Bacterial

Mutation

WWE domain deletion

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-EGFP-mPkm2

Plasmid

#242812

Purpose

Lentiviral vector expressing mouse Pkm2 tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

pyruvate kinase, muscle (Pkm Mouse)

Use

Lentiviral

Tags

EGFP

Promoter

Ubiquitin C

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ER-GFP pLVX CMVpuro

Plasmid

#246912

Purpose

Lentiviral-based expression of EGFP with an ER retention signal sequence in mammalian cells

Depositor

William Kaelin

Article

Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.mIRFP670nano3

Plasmid

#244107

Purpose

Bacterial expression of green glucose sensor with inert mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Tags

mIRFP670nano3

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr201 RNF146 deltaRING

Plasmid

#240332

Purpose

Entry vector for Gateway with RNF146 (RING domain deletion)

Depositor

Junjie Chen

Article

Nie et al Mol Cell Proteomics. 2020 Dec;19(12):201

Insert

RNF146 (RNF146 Human)

Expression

Bacterial

Mutation

RING domain deletion

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSB02

Plasmid

#230991

Purpose

B. dendrobatidis plasmid for integration of target sequence at URA3 locus, blasticidin S selection

Depositor

Lillian Fritz-Laylin

Article

Brody et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

BdURA3_LHA2-Hsbsr-ScADH1ter

Use

Bd gene targeting vector

Tags

bsr

Expression

Bacterial

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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EBFP2-Kindlin2-3KA

Plasmid

#242823

Purpose

Encodes human kindlin2-3KA mutant and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Kindlin2 (FERMT2 Human)

Expression

Mammalian

Mutation

K385A, K386A, K393A

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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EBFP2-K1K2

Plasmid

#242819

Purpose

Encodes human kindlin1 containing kindlin2 F3 domain and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Kindlin1 (Fermt1 Human)

Expression

Mammalian

Mutation

Kindlin1 chimera containing the F3 domain of kind…

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_MGA

Plasmid

#240327

Purpose

Entry vector for Gateway with MGA

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

MGA (MGA Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-Gnai3

Plasmid

#245997

Purpose

Express HA N-terminal tagged mouse Galphai3 transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnai3 (Gnai3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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