We narrowed to 63,901 results for: cloning
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Plasmid#82596Purpose3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (W2101F) without pA for rapamycin/rapalog induced dimerizationDepositorInsertFRB-HA (no pA)
MutationW2101FPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS cSugarCrisp (CT#708)
Plasmid#13956DepositorPublicationInsertSugarCrisp (PI15 Chicken)
ExpressionBacterialAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS hSugarCrisp (CT#592)
Plasmid#13966DepositorPublicationInsertSugarCrisp (PI15 Human)
ExpressionBacterialAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pdCRISPomyces-2BD
Plasmid#234429PurposePlasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning templateDepositorInsertModified dCas9(D10A, H840A) with polyaspartate linking
ExpressionBacterialMutationLinked polyaspartate using glycine-serine linkerAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-hU6-evopreQ1
Plasmid#210189PurposepegRNA/ngRNA library cloningDepositorTypeEmpty backboneUseLentiviralAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)
Plasmid#75412PurposeModule for the expression of the Renilla Luciferase with the silencing suppressor P19DepositorInsertRenilla / P19
UseLuciferase and Synthetic BiologyExpressionPlantPromoter35SAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTK_BsmBI_LacZ_Citrine_Nanotag_4
Plasmid#130514PurposeEnhancer cloning nanotag reporter vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLacZ
UseEnhancer cloning nanotag reporter vectorPromoterthymidine kinase minimal promoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBP-J23100
Plasmid#72963PurposeLevel 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)DepositorPublicationInsertJ23100 promoter
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-THT III
Plasmid#55791PurposeGATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Gentamycin resistanceDepositorTypeEmpty backboneUseGateway Cloning and RNAiExpressionMammalianPromotermodified H1 RNA geneAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA
Plasmid#171384PurposeExpression vector for encoding a human codon-optimized dCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized dCas13X.1-RESCUE-S
ExpressionMammalianPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC5GGv1
Plasmid#194645PurposepSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionExpressionBacterial and YeastAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEntry_HCoV-229E-Spike
Plasmid#168971PurposeGateway-compatible Entry vectorDepositorInsertHCoV-229E-Spike (S )
UseGateway CloningAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-3xFLAG-hSpCas9
Plasmid#162161PurposeExpresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTags3xFLAGExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAGM55285
Plasmid#153212PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJMP1354
Plasmid#119275Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
MZB184
Plasmid#228488PurposeEmpty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneTags10xHis-SUMO(Smt3)ExpressionBacterialPromoterT7 PromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
C78m
Plasmid#120990PurposeMoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.DepositorInsertChlorR
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCENTR_aco_mScarlet3
Plasmid#225984PurposeArabidopsis codon-optimized mScarlet3 in an entry cloneDepositorInsertmScarlet3 Arabidopsis codon-optimized
UseSynthetic BiologyMutationArabidopsis Codon-Optimized mScarlet3Available sinceOct. 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX458_Ruby
Plasmid#110164PurposeCas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthSpCas9
UseCRISPRTags3xFLAG and Ruby2ExpressionMammalianAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pgRGFP
Plasmid#82695PurposeExpresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterPGK, U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by