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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,291 results for: grna

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Showing: 10501 - 10520 of 16291 results

  1. pJAT31

    Plasmid
    #204291
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-DsRed
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204291-plasmid-map-sequence-id-405841

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  2. pRGEB31

    Plasmid
    #51295
    Purpose
    Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.
    Depositor
    Yinong Yang
    Article
    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    Rice snoRNA U3 and dual 35S promoter
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    51295-plasmid-map-sequence-id-339890

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  4. TRMPVIR

    Plasmid
    #27994
    Purpose
    Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.
    Depositor
    Scott Lowe
    Article
    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.
    Insert
    TRMPVIR (shRen)
    Use
    RNAi and Retroviral; Fluorescent reporters
    Expression
    Mammalian
    Available Since
    March 18, 2011
    Availability
    Academic Institutions and Nonprofits only
    27994-plasmid-map-sequence-id-13249

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  7. pADH99

    Plasmid
    #90979
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    US_HIS1
    FRT
    pENO1
    C. albicans Cas9
    pMAL2
    FLP
    NAT 1 of 2
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90979-plasmid-map-sequence-id-180505

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  17. pCAG-EGxxFP

    Plasmid
    #50716
    Purpose
    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.
    Depositor
    Masahito Ikawa
    Article
    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103
    Insert
    split EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    50716-plasmid-map-sequence-id-447281

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  20. GB4070

    Plasmid
    #187811
    Purpose
    Module for the constitutive expression of Cup2:Gal4AD and gRNA-1 DFR, and the copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).
    Depositor
    Diego Orzaez
    Article
    Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.
    Insert
    copper-inducible dCasEV2.1
    Expression
    Plant
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    187811-plasmid-map-sequence-id-378054

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Showing: 10501 - 10520 of 16291 results