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pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

Plasmid

#223159

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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Slc17a7-IRES2-Cre targeting vector

Plasmid

#61574

Purpose

Target the Cre recombinase gene to the stop codon of the mouse Slc17a7 (VGlut1) gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Slc17a7-IRES2-Cre

Use

Cre/Lox and Mouse Targeting

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65455

Purpose

Can be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Promoter

CAG

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCIG-VP16–RAR-alpha

Plasmid

#80434

Purpose

Expression of constitutively active human RAR-alpha in chick embryos

Depositor

Aixa Morales

Article

Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

Insert

Retinoic acid receptor alpha (RARA Human)

Tags

EGFP and VP16

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only
Pvalb-2A-Flpo Flp-in replacement vector

Plasmid

#61572

Purpose

Recombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb-2A-Flpo

Use

Recombinase-mediated cassette exchange using flp …

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pBK1303-AAV-EFSNC-dCjCas9-MECP2(204-310)

Plasmid

#223148

Purpose

Expression of truncated MECP2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310

Promoter

EF1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Snap25-LSL-2A-GFP targeting vector

Plasmid

#61575

Purpose

Target a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Snap25-LSL-2A-GFP

Use

Cre/Lox and Mouse Targeting

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65453

Purpose

Can be used to generate AAV virus that will express EGFP from the tetO promoter under intersectional control by Flp and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV1

Insert

EGFP

Use

AAV

Promoter

tetO

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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BCL6-GFP (1-275AA) in Cilantro

Plasmid

#169932

Purpose

Overexpression of 1-275AA BCL6-GFP fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 1-275AA (BCL6 Human)

Use

Lentiviral

Tags

GFP

Mutation

160R>C

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1-1605-shRNAres_W

Plasmid

#147889

Purpose

Mammalian Expression of HsNot1iso1_1-1605-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1-1605-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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