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pCK014

Plasmid

#105346

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Tet-on-vsv-Incenp

Plasmid

#108480

Purpose

inducible expression of vsv-INCENP wt

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch12_62418577-gRNA-rev

Plasmid

#81217

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch12 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCALNL_Ch10

Plasmid

#81224

Purpose

pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in PCDH15 locus on chromosome 10

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

gix-Neo-gix deletion cassette upstream of EGFP

Expression

Mammalian

Promoter

pCBa

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rNanog-pgk-hph

Plasmid

#74917

Purpose

Mammalian expression of rNanog

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Rat)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pUPD_OsU3 (GB1184)

Plasmid

#68209

Purpose

Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

OsU3 promoter

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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p6xUAS (GB0179)

Plasmid

#68182

Purpose

Provides 6xUAS (upstream activating sequence) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

6xUAS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pJB045 (ZapB-mEos2)

Plasmid

#72651

Purpose

Inducible expression of ZapB-mEos2 in bacteria

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

ZapB-mEos2

Tags

mEos2

Expression

Bacterial

Promoter

T5-lac

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pT35S (GB0036)

Plasmid

#68187

Purpose

Provides the CaMV 35S terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

CaMV 35S terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pYFP (GB0053)

Plasmid

#68197

Purpose

Provides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

YFP coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pP[RS3]3'M

Plasmid

#53552

Purpose

for an easy screen of TALEN- and CRISPR/Cas9- mediated mutagenesis in Drosophila

Depositor

Renjie Jiao

Article

Yu et al Biol Open. 2014 Apr 15;3(4):271-80. doi:

Insert

AscI and MluI restriction enzyme sites

Expression

Insect

Promoter

same pP[RS3]

Available Since

Dec. 3, 2014

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C3_9

Plasmid

#60258

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

NKX6-1 enhancer (NKX6-1 Human)

Use

Gateway Cloning

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_25

Plasmid

#60268

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ROCK1 enhancer (ROCK1 Human)

Use

Gateway Cloning

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_7

Plasmid

#60293

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SLC30A8 enhancer (SLC30A8 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_25

Plasmid

#60305

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ROCK1 enhancer (ROCK1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
CtkA_300_cmv-10

Plasmid

#27434

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

3xFLAG

Expression

Mammalian

Mutation

residues 301-325 are truncated

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D155Q/D179Q_cmv-10

Plasmid

#27435

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

3xFLAG

Expression

Mammalian

Mutation

D155Q/D179Q

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pBS SDF1

Plasmid

#19202

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

SDF1 (CXCL12 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
7852 pUG72-HSC82out

Plasmid

#10895

Depositor

Susan Lindquist

Article

Cowen et al Science. 2005 Sep 30. 309(5744):2185-9.

Insert

HSC82 (HSC82 Budding Yeast)

Use

Cre/Lox

Expression

Yeast

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only

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HaloTag-eGFP-OMP25(MTS)

Plasmid

#188661

Purpose

HaloTag-GFP fusion targeted to mitochondria with OMP25 targeting sequence.

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

GFP-HALO-OMP25(MTS)

Expression

Mammalian

Promoter

Chicken beta actin

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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