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S1_AscI_V5-gateway_Bsu36I

Plasmid

#128467

Purpose

destination vector with N-terminal V5 tag

Depositor

Lindy Barrett

Publication

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

destination vector with N-terminal V5 tag

Expression

Bacterial

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S2_AscI_HA-gateway_Bsu36I

Plasmid

#128468

Purpose

destination vector with N-terminal HA tag

Depositor

Lindy Barrett

Publication

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

destination vector with N-terminal HA tag

Expression

Bacterial

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-21

Plasmid

#104707

Purpose

Cloning Module

Depositor

Marco Trujillo

Publication

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

PUB22 WT (PUB22 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Rap1b S179AS180A

Plasmid

#118326

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Publication

Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

Insert

Rap1b S179AS180A (RAP1B Bovine)

Tags

Flag

Expression

Mammalian

Mutation

S179AS180A

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEF1a-CRY2PHR-SID4X

Plasmid

#63663

Purpose

AAV expression of CRYS2PHR-SID4X

Depositor

Dong Kong

Publication

Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

Insert

SID4X-CYR2PHR

Use

AAV

Expression

Mammalian

Promoter

EF1alpha

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG301

Plasmid

#105358

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promotor DD45/EC1.2 (At2g21740;1000bp)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMDC32-FIT2

Plasmid

#96990

Purpose

Express mouse FIT2 gene in plants.

Depositor

Kent Chapman

Publication

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Expression

Plant

Promoter

35S

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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beta4-nAChR shRNA (Beta-4.3)

Plasmid

#86650

Purpose

expression of shRNA targeting CHRNB4

Depositor

Jaime Modiano

Publication

Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

Insert

CHRNB4 (beta4-nAChR) (CHRNB4 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pHU6_gRNA_Ch10-for1

Plasmid

#81210

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 3' of gix site)

Depositor

David Liu

Publication

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_gRNA_Ch10-rev1

Plasmid

#81208

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 5' of gix site)

Depositor

David Liu

Publication

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pTNos:BASTA:PNos (GB0023)

Plasmid

#68211

Purpose

Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

bar gene

Use

Unspecified

Mutation

BsaI and BsmBI sites removed

Promoter

PNos

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_31

Plasmid

#60310

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Publication

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SLC2A2 enhancer (SLC2A2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-HIRAmut-polyA

Plasmid

#59782

Purpose

This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).

Depositor

Yi Zhang

Publication

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Expression

Mammalian

Mutation

Change from 'GATCGAAGTTTG' to 'GAc…

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pFF19H

Plasmid

#49783

Purpose

expresses hygromycin resistance gene in plant cells

Depositor

Joachim Messing

Publication

Timmermans et al J Biotechnol. 1990 Jun;14(3-4):333-44.

Insert

HPH

Use

Plant expression

Promoter

CaMV 35S

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only
pICH71431

Plasmid

#50344

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

3'UTR, polyadenylation signal/terminator, ATPase (S. lycopersicum )

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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CS2-Myc-mASUN

Plasmid

#47044

Purpose

Expression of tagged-mouse ASUN

Depositor

Laura Lee

Publication

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Asunder (Asun Mouse)

Tags

Myc

Expression

Mammalian

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
pFM115

Plasmid

#40161

Depositor

Geraldine Seydoux

Publication

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3C (par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

C terminal portion of protein (aa850-1286)

Promoter

tac

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pBS FAM20C

Plasmid

#19214

Depositor

Cliff Tabin

Publication

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

FAM20C (FAM20C Chicken)

Expression

Bacterial

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS ALS2

Plasmid

#19228

Depositor

Cliff Tabin

Publication

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Amyotrophic lateral sclerosis 2 (juvenile) (ALS2 Chicken)

Expression

Bacterial

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
RCAS (A) Lunatic Fringe (CT#482)

Plasmid

#13977

Depositor

Cliff Tabin

Publication

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Lunatic Fringe (LFNG Chicken)

Use

Retroviral; Avian expression

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only