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pFA6a-pcr1P-natMX6-pcr2P

Plasmid

#80620

Purpose

to delete part of the mat1-P locus and fix the mating type in natural S. pombe isolates

Depositor

Miguel Godinho Ferreira

Article

Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

Inserts

PCR1 from mat1-P
PCR2 from mat1-P

Expression

Yeast

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-pcr1M-natMX6-pcr2M

Plasmid

#80621

Purpose

to delete part of the mat1-M locus and fix the mating type in natural S. pombe isolates

Depositor

Miguel Godinho Ferreira

Article

Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

Inserts

PCR1 from mat1-M
PCR2 from mat1-M

Expression

Yeast

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAWK

Plasmid

#62713

Purpose

Donor vector for amiR-eGFP knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123/amiR-eGFP419/FLPe

Use

RNAi

Expression

Mammalian

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pARD (pKD-G4)

Plasmid

#62683

Purpose

Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP419

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pET11a+T214V

Plasmid

#66134

Purpose

Expresses the T214V mutant of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Expression

Bacterial

Mutation

T214V

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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mRFP1-19

Plasmid

#63820

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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mRFP1-35

Plasmid

#63824

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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mRFP1-31

Plasmid

#63823

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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mRFP1-29

Plasmid

#63822

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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mRFP1-21

Plasmid

#63821

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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mRFP1-18

Plasmid

#63819

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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mRFP1-8

Plasmid

#63817

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCri-4a-Strep

Plasmid

#61338

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag, Strep-tag, and TRX-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pFloxin-IRES-Ofd1-Myc-S75F

Plasmid

#24574

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Mutation

serine 75 changed to phenylalanine (codon TCT cha…

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pFloxin-IRES-Ofd1-Myc-A80T

Plasmid

#24575

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Mutation

Alanine 80 changed to threonine (codon GCA to ACA)

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pFloxin-IRES-Ofd1-Myc-G139S

Plasmid

#24576

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Mutation

Glycine 139 changed to serine (codon GGT to AGT)

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pFloxin-Ofd1-Myc-S437R

Plasmid

#24577

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Mutation

Serine 437 changed to Arginine (codon AGT to CGT)…

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-DR RecR(K23A/R27A)

Plasmid

#12619

Depositor

SeWon Suh

Article

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR (recR Deinococcus radiodurans)

Tags

His

Expression

Bacterial

Mutation

changed Lysine 23 to Alanine, and Arginine 27 to …

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pMMTV neu NT

Plasmid

#1823

Depositor

Philip Leder

Article

Muller et al Cell 1988 Jul 1;54(1):105-15.

Insert

neu NT (Erbb2 Rat)

Expression

Mammalian

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
L1_HyR-Ck1

Plasmid

#136125

Purpose

L1 in position 1 (next to LB in L2 construct), for HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35S:hptII_3t35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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