Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 16,278 results for: nes

Clear keywords
Showing: 10561 - 10580 of 16278 results

  7. DCT.VV.Orf117

    Plasmid
    #12805
    Purpose
    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.
    Depositor
    Jack Bennink, Jon Yewdell
    Publication
    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VV.Orf117
    Expression
    Mammalian
    Available since
    Dec. 8, 2006
    Availability
    Academic Institutions and Nonprofits only

  9. TRE3GV-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid
    #222508
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; C706A and R832…
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222508-plasmid-map-sequence-id-441225

    Enlarge

  10. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid
    #61361
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61361-plasmid-map-sequence-id-99794

    Enlarge

  11. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

    Enlarge

  13. pGIPZ EF1a spIgE-3xFLAG-Neo2/15 IRES mNeonGreen-P2A-Puro

    Plasmid
    #248034
    Purpose
    Constitutively expresses 3xFLAG tagged Neoleukin 2/15 with an IgE signal peptide, mNeonGreen and a puromycin resistance gene in human cells
    Depositor
    Joshua Leonard
    Publication
    Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.
    Insert
    3xFLAG-tagged Neoleukin-2/15
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    human EF1a
    Available since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248034-plasmid-map-sequence-id-497728

    Enlarge

  14. pIDTSmart-CMV-PLRS1

    Plasmid
    #217365
    Purpose
    Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"
    Depositor
    Abhishek Chatterjee
    Publication
    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    leucyl-tRNA synthetase polyspecific mutant
    Expression
    Mammalian
    Mutation
    M40I, T252A, Y499I, Y527A, H529G
    Promoter
    CMV
    Available since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    217365-plasmid-map-sequence-id-430948

    Enlarge

  15. CaMK2rep-S603A

    Plasmid
    #239623
    Purpose
    Inactive CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.
    Depositor
    F Javier Díez Guerra
    Publication
    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpotNES-sGFP2-SynP3-3xmyc (Syn1 Synthetic, Rat)
    Tags
    Spot, myc, and sGFP2
    Expression
    Mammalian
    Mutation
    changed Serine 603 to Alanine
    Promoter
    CMV
    Available since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    239623-plasmid-map-sequence-id-476237

    Enlarge

  16. AiP13657 - pAAV-AiE0655m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3657)

    Plasmid
    #230163
    Purpose
    AiE0655m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Promoter
    minBG
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    230163-plasmid-map-sequence-id-461888

    Enlarge

  17. AiP13635 - pAAV-AiE0387m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3635)

    Plasmid
    #230159
    Purpose
    AiE0387m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Promoter
    minBG
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    230159-plasmid-map-sequence-id-461884

    Enlarge

  18. AiP13294 - pAAV-AiE0940m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3294)

    Plasmid
    #230144
    Purpose
    AiE0940m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Promoter
    minBG
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    230144-plasmid-map-sequence-id-461871

    Enlarge

  19. AiP13295 - pAAV-AiE0941m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3295)

    Plasmid
    #230145
    Purpose
    AiE0941m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Publication
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Promoter
    minBG
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    230145-plasmid-map-sequence-id-461872

    Enlarge

Showing: 10561 - 10580 of 16278 results