Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 14,072 results for: CRISPR-Cas9

Clear keywords
Showing: 1041 - 1060 of 14072 results

  13. evoCas9

    Plasmid
    #138558
    Purpose
    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    evoCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138558-plasmid-map-sequence-id-274774

    Enlarge

  15. HeFm2SpCas9

    Plasmid
    #92111
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    “Highly enhanced Fidelity” mut2 SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, R1060A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92111-plasmid-map-sequence-id-197616

    Enlarge

  16. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid
    #217304
    Purpose
    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter
    Depositor
    Luke Gilbert
    Insert
    dCas9-tagBFP-KRAB
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Promoter
    Ef1alpha
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217304-plasmid-map-sequence-id-429717

    Enlarge

  17. pTRE3G-VP64-dCas9-VP64-ZsG

    Plasmid
    #192668
    Purpose
    Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    VP64-dCas9-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    TRE3G
    Available Since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192668-plasmid-map-sequence-id-379892

    Enlarge

  18. H1_sgRNA(GRIN2B)_SauCas9_AcrIIA5-AsLOV2(N77)

    Plasmid
    #246056
    Purpose
    Expression of a sgRNA targeting the GRIN2B locus, a SauCas9 and an AcrIIA5-cpGR2 hybrid (insertion before N77) linked through a P2A sequence, under the same pol-III H1 promoter
    Depositor
    Dominik Niopek
    Article
    Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk
    Insert
    SauCas9, sgRNA(GRIN2B) and AcrIIA5_AsLOV2-N77
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    246056-plasmid-map-sequence-id-495342

    Enlarge

  19. H1_sgRNA(GRIN2B)_SauCas9_AcrIIA5-AsLOV2(D41)

    Plasmid
    #246055
    Purpose
    Expression of a sgRNA targeting the GRIN2B locus, a SauCas9 and an AcrIIA5-cpGR2 hybrid (insertion before D41) linked through a P2A sequence, under the same pol-III H1 promoter
    Depositor
    Dominik Niopek
    Article
    Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk
    Insert
    SauCas9, sgRNA(GRIN2B) and AcrIIA5_AsLOV2-D41
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    246055-plasmid-map-sequence-id-495341

    Enlarge

  20. Lenti Cas9/SB100

    Plasmid
    #155294
    Purpose
    Lentiviral expression of Cas9 and SB100
    Depositor
    Martin Carroll
    Insert
    SB100X-P2A-spCas9
    Expression
    Mammalian
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155294-plasmid-map-sequence-id-309998

    Enlarge

Showing: 1041 - 1060 of 14072 results