We narrowed to 9,669 results for: Coli
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Plasmid#82040PurposeHybrid NarQ[1-217]-Tar[257-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and histidine kinase NarQ: NarQ[1-217]-Tar[257-553]
UseTagsExpressionMutationPromoterAvailable SinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK501
Plasmid#72246PurposeProduces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationGln206 to Arg and changes tryptophan-151 to pheny…PromoterT7 and endogenousAvailable SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK500
Plasmid#72245PurposeProduces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationPro64 to Arg; Asp65 to His; Gln206 to Arg and cha…PromoterT7 and endogenousAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL 1571
Plasmid#37760DepositorInsertsptet_ompC_gfpAaV
pLac_st3 TetR_mCherry
UseSynthetic Biology; E. coliTagsgfp and mCherryExpressionMutationAaV degradation tag added to the 3'endPromoterpLac and ptetAvailable SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL 1569
Plasmid#37759DepositorInsertsptet_ompC_gfpAaV
pLac_micC_mCherry
UseSynthetic Biology; E. coliTagsgfp and mCherryExpressionMutationAaV degradation tag added to the 3'endPromoterpLac and ptetAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterNative promoter from pMUT2 relB/relE operon and P…Available SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-AT
Plasmid#133306Purpose"Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisationDepositorInsertaxe/txe toxin-antitoxin cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRelA*
Plasmid#175590PurposeTet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
UseTagsFused with a flexible GS linker to mVenusExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…PromoterAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-HS
Plasmid#133305Purpose"Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisationDepositorInserthok/sok toxin-antitoxin cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD-SepRS-EFSep
Plasmid#34623DepositorUseTagsExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterPtac and Ptac (from SepRS- operon like)Available SinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMis1_1B
Plasmid#192079PurposeA pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent inductionDepositorInsertsKanR
rhaR
rhaS
pBBR1 rep
UseTagsExpressionBacterialMutationPromoterAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM6-FAS
Plasmid#49810PurposeEncodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configurationDepositorInsertsfatty acyl-ACP thioesterase
hydroxydecanoyl-ACP dehydratase
b-ketoacyl-ACP synthase III
3-ketoacyl-ACP reductase
enoyl-(acyl carrier protein) reductase
UseTagsExpressionBacterialMutationCodon-optmized. See supplemental information of …PromoterT7Available SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMA7
Plasmid#79967PurposeTM-MAGE strainDepositorInsertsLambda Red recombinase beta subunit
DNA adenine methylase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYihI-N+C-term K-R
Plasmid#233093PurposeExpression of GST-YihI with N+C-term lysines (K) mutated to arginine (R)DepositorInsertGST-YihI with N+C-term lysines (K) mutated to arginine (R)
UseTagsGSTExpressionBacterialMutationGST-YihI with N+C-term lysines (K) mutated to arg…PromoterAvailable SinceMay 12, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRAR101
Plasmid#185046PurposeJ23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region with amino acids 2-8 deleted, trrnBDepositorInsertL31p
UseTagsExpressionBacterialMutationAmino acids 2-8 deleted of L31p coding regionPromoterAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR111
Plasmid#185047PurposeJ23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region with last 8 amino acids deleted, trrnBDepositorInsertL31p
UseTagsExpressionBacterialMutationLast 8 amino acids deleted of L31p coding regionPromoterAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR120
Plasmid#185041PurposeJ23108 promoter, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationOnly first 90 nt of rpmE coding presentPromoterAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-Ala2
Plasmid#71326PurposeNADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterinsulated waaHp from E. coliAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVR2
Plasmid#84719PurposeE. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcriptionDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutation12 nt stretch lacking A's inserted before 5&…PromoterAvailable SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ala-eK-Flv
Plasmid#83393Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only