We narrowed to 9,485 results for: Coli

1. pKP997

   Plasmid

   #167656

   Purpose

   Express colicinB-His6, V20C, M261C

   Depositor

   Kathleen Postle

   Insert

   cba-his6 V20C, M261C
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   cba-his6 with V20C, M261C mutations

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKP2264

   Plasmid

   #167660

   Purpose

   Express colicinB-His6, A121C, Y254C

   Depositor

   Kathleen Postle

   Insert

   cba-his6, A121C, Y254C
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   cba-his6, I121C, Y254C

   Promoter

   Available since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBT1-proD-mCherry

   Plasmid

   #65823

   Purpose

   Const. mCherry expression , proD promoter, E. coli Expression

   Depositor

   Michael Lynch

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   proD E . coli Constitutive Promoter

   Available since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZH_tdPP7cp_mCherry

   Plasmid

   #118267

   Purpose

   Low-noise, tetracycline inducible expression of tdPP7cp-mCherry

   Depositor

   Zach Hensel

   Insert

   tdPP7cp-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-TALEN-Rep

   Plasmid

   #45964

   Depositor

   Ralf Kuehn

   Insert

   beta-Galactosidase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   duplicated aa 1-135

   Promoter

   CMV

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZH_mPP7cp_mCerulean3

   Plasmid

   #118262

   Purpose

   Low-noise, tetracycline inducible expression of mPP7cp-mCerulean3

   Depositor

   Zach Hensel

   Insert

   mPP7cp-mCerulean3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZH_tdPP7cp_mCerulean3

   Plasmid

   #118263

   Purpose

   Low-noise, tetracycline inducible expression of tdPP7cp-mCerulean3

   Depositor

   Zach Hensel

   Insert

   tdPP7cp-mCerulean3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZH_mPP7cp_mCherry

   Plasmid

   #118266

   Purpose

   Low-noise, tetracycline inducible expression of mPP7cp-mCherry

   Depositor

   Zach Hensel

   Insert

   mPP7cp-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZH_tdPP7cp_ECFP

   Plasmid

   #118265

   Purpose

   Low-noise, tetracycline inducible expression of tdPP7cp-ECFP

   Depositor

   Zach Hensel

   Insert

   tdPP7cp-ECFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZH_mPP7cp_ECFP

    Plasmid

    #118264

    Purpose

    Low-noise, tetracycline inducible expression of mPP7cp-ECFP

    Depositor

    Zach Hensel

    Insert

    mPP7cp-ECFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-Rab-Rep

    Plasmid

    #45967

    Depositor

    Ralf Kuehn

    Insert

    beta-Galactosidase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    duplicated aa 1-135

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA C12 ecDHFR DD YFP HA

    Plasmid

    #192815

    Purpose

    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

    Depositor

    John Hulleman

    Article

    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

    Insert

    E. coli dihydrofolate reductase
    

    Use

    Tags

    enhanced yellow fluorescent protein and hemagglut…

    Expression

    Mammalian

    Mutation

    W74R/T113S/E120D/Q146L

    Promoter

    CMV

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEM-Cas9HF1-recA56

    Plasmid

    #89962

    Purpose

    Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Inserts

    Cas9HF1
    recA56
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet and proD

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJBEI-6409

    Plasmid

    #47048

    Purpose

    BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    

    Use

    Synthetic Biology; Bglbrick

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacUV5

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJBEI-6410

    Plasmid

    #47049

    Purpose

    BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    

    Use

    Synthetic Biology; Bglbrick

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacUV5

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC-GFP-MCC

    Plasmid

    #133307

    Purpose

    "Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation

    Depositor

    Chris Barnes

    Article

    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

    Insert

    microcin-V bacteriocin cassette (cvaC E. coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    N112D (please see depositors comments)

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPReporter-mCherry

    Plasmid

    #65008

    Purpose

    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    User-defined

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSF-Ivy-his-hLYZ

    Plasmid

    #83920

    Purpose

    Co-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Ivy
    lysozyme (LYZ Human)
    

    Use

    Tags

    Ivy protein has C-terminal hisx6 tag

    Expression

    Bacterial

    Mutation

    Ivy leader sequence deleted and replaced.

    Promoter

    T7 and t7

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM6-RgTALsyn-HpaBC

    Plasmid

    #100949

    Purpose

    Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Inserts

    RgTALsyn
    HpaB
    HpaC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSMART-ala10

    Plasmid

    #87135

    Purpose

    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Inserts

    NADPH Alanine Dehydrogenase (ald Synthetic)
    alanine exporter (alaE Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D196A, L197R

    Promoter

    waaHp from E. coli as part of operon with ald* an…

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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