We narrowed to 4,746 results for: MLS

1. pEGIP*35

   Plasmid

   #26776

   Purpose

   Enables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targeting

   Depositor

   Linzhao Cheng

   Article

   Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

   Insert

   GFP*35
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDR366 RNF2

   Plasmid

   #47509

   Purpose

   human gRNA expression vector targeting RNF2

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-RNF2 (RNF2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mADAM10 E385A in pQCXIP cFLAG

   Plasmid

   #19137

   Depositor

   Zena Werb

   Article

   Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

   Insert

   ADAM10 (Adam10 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E385A

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pVC299 VEGF Site#2

   Plasmid

   #47506

   Purpose

   human gRNA expression vector targeting VEGF

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-VEGF site 2 (VEGFA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-(pos36)GFP-9xGGS-Cre-6xHis

   Plasmid

   #62938

   Purpose

   Expression of (+36)GFP-GGS9-Cre-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (ps36)GFP-9xGGS-Cre
   

   Use

   Tags

   (pos36)GFP and 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCerulean3-Golgi-7

   Plasmid

   #55420

   Purpose

   Localization: GalT Targeting Sequence, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   Golgi (B4GALT1 Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   aa 1-82 of NM_001497.3

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

7. YFP-MEKK3

   Plasmid

   #12189

   Depositor

   Gary Johnson

   Article

   Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

   Insert

   MEKK3 (Map3k3 Mouse)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pRibo-Sec-APEX2m

   Plasmid

   #176844

   Purpose

   Expression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-Sec-APEX2m
   

   Use

   Inducible

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HOXA4 (murine) HA-tag pET

   Plasmid

   #8489

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXA4 (Hoxa4 Mouse)
   

   Use

   Tags

   HA and T7

   Expression

   Bacterial

   Mutation

   Thr-2 changed to Ala-2 to form better ribosomal b…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pETSUMO2_Rhodoplanes-bGSDM

    Plasmid

    #212129

    Purpose

    For expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Science. 2022 Jan 14;375(6577):221-225.

    Insert

    Rhodoplanes bGSDM
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETSUMO2_Vitiosangium-bGSDM

    Plasmid

    #212127

    Purpose

    For expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Science. 2022 Jan 14;375(6577):221-225.

    Insert

    Vitiosangium bGSDM
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETSUMO2_Gillisia-bGSDM

    Plasmid

    #212128

    Purpose

    For expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Science. 2022 Jan 14;375(6577):221-225.

    Insert

    Gillisia bGSDM
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETSUMO2_Bradyrhizobium-bGSDM

    Plasmid

    #212126

    Purpose

    For expression of the bacterial gasdermin (bGSDM) encoded by a Bradyrhizobium species. Protein is expressed with an N-terminal SUMO2 tag.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Science. 2022 Jan 14;375(6577):221-225.

    Insert

    Bradyrhizobium bGSDM
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV-REPORT (PGK) 5xGRE-NanoLuc

    Plasmid

    #207170

    Purpose

    GAl4 response element driven NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Roennfeldt et al Biomolecules. 2023 Oct 19;13(10):1545. d

    Inserts

    NanoLuciferase
    d2eGFP
    5x GRE
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Expression

    Mutation

    Promoter

    Gal4 response element minP and PGK

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCerulean3-Fibrillarin-7

    Plasmid

    #55419

    Purpose

    Localization: Nucleoli, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

16. mADAM10 deltaMP in pQCXIP cFLAG

    Plasmid

    #19138

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM10 (Adam10 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    delta 56-457

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pPICZ-mouseGIRK2-eGFP-StII-6xHis

    Plasmid

    #124277

    Purpose

    Expresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

    Depositor

    Arthur Laganowsky

    Article

    Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

    Insert

    GIRK2 (Kcnj6 Mouse)
    

    Use

    Tags

    eGFP-StreptagII-6xHis

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCerulean3-LaminB1-10

    Plasmid

    #55425

    Purpose

    Localization: Nuclear Envelope, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pVC297 VEGF Site#1

    Plasmid

    #47505

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 1 (VEGFA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HOXD4 (murine) MSCV

    Plasmid

    #8549

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXD4 (Hoxd4 Mouse)
    

    Use

    Retroviral

    Tags

    His and T7

    Expression

    Mammalian

    Mutation

    contains T7 and HIS tags from pET; makes protein …

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only