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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 1041 - 1060 of 4467 results

  3. pJL1-MBP-4xDQNAT

    Plasmid
    #128390
    Purpose
    In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Maltose binding protein
    Tags
    4xDQNAT glycosylation tag and 6xHis tag
    Expression
    Bacterial
    Mutation
    A302T- please see depositor comment
    Promoter
    T7
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128390-plasmid-map-sequence-id-260183

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  10. pAGW-dpse-GAL4-DBD

    Plasmid
    #125153
    Purpose
    Gateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CP
    Depositor
    Alexander Stark
    Article
    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:
    Insert
    D. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
    Use
    Gateway fly expression vector
    Expression
    Insect
    Available Since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    125153-plasmid-map-sequence-id-248016

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  11. pSTAP-seq_fly-zfh1

    Plasmid
    #86381
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer
    Use
    Stap-seq screening vector
    Available Since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86381-plasmid-map-sequence-id-173935

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  14. pSTAP-seq_fly-tj

    Plasmid
    #86386
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster tj enhancer
    Use
    Stap-seq screening vector
    Available Since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86386-plasmid-map-sequence-id-173933

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  17. pSTAP-seq_fly-4xUAS

    Plasmid
    #125149
    Purpose
    Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells
    Depositor
    Alexander Stark
    Article
    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:
    Insert
    4 x upstream activating sequence (UAS)
    Use
    Stap-seq screening vector
    Available Since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    125149-plasmid-map-sequence-id-247994

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  20. pSTAP-seq_fly-ssp3

    Plasmid
    #86382
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster ssp3 enhancer
    Use
    Stap-seq screening vector
    Available Since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86382-plasmid-map-sequence-id-173943

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Showing: 1041 - 1060 of 4467 results