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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,270 results for: Streptococcus

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Showing: 1041 - 1060 of 3270 results

  2. pMZ381

    Plasmid
    #59899
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469
    Depositor
    Mikel Zaratiegui
    Publication
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59899-plasmid-map-sequence-id-94872

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  3. HypaCas9

    Plasmid
    #138557
    Purpose
    Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    HypaCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N692A, M694A, Q695A, H698A
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138557-plasmid-map-sequence-id-274767

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  5. PX394 Neisseria cinerea Cas9

    Plasmid
    #68331
    Purpose
    Neisseria cinerea Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68331-plasmid-map-sequence-id-119554

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  6. pSc1

    Plasmid
    #80436
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Publication
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80436-plasmid-map-sequence-id-151445

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  7. TMPRSS2 g1 + Cas9

    Plasmid
    #153015
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153015-plasmid-map-sequence-id-298676

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  10. pCM4.4

    Plasmid
    #217648
    Purpose
    an ermE*p-cas9-driven expression, as an alternative plasmid
    Depositor
    Huimin Zhao
    Publication
    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon Optimized SpCas9
    Use
    CRISPR
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217648-plasmid-map-sequence-id-434957

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  11. B-HiFi SpCas9

    Plasmid
    #207362
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-HiFi SpCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    R691A, amino acids 1005-1013 replaced with two gl…
    Promoter
    CBh
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207362-plasmid-map-sequence-id-410135

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  12. B-Sniper SpCas9

    Plasmid
    #207361
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207361-plasmid-map-sequence-id-410129

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  19. pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)

    Plasmid
    #160647
    Purpose
    module containing the human codon optimized Cas9 and the BASTA selection marker
    Depositor
    Diego Orzaez
    Insert
    BASTA / Cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, P35S
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160647-plasmid-map-sequence-id-319125

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Showing: 1041 - 1060 of 3270 results