We narrowed to 9,891 results for: plasmids 101

1. 6CC8 (MBD3)

   Plasmid

   #119959

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   MBD3 (MBD3 Human)
   

   Use

   Tags

   GSTtag, 6xHis and Thrombin

   Expression

   Bacterial

   Mutation

   MBD domain (contains residues 1-71)

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pMJH65

   Plasmid

   #67273

   Purpose

   Conjugally transferable red recombinogenic plasmid

   Depositor

   Mark Liles

   Article

   Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

   Insert

   tetA
   

   Use

   Red recombinase, temperature sensitive

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetA promoter of plasmid pACYC184 origin

   Available since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJUMP45-2A(sfGFP)

   Plasmid

   #126989

   Purpose

   Level 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJUMP25-1A(sfGFP)

   Plasmid

   #126972

   Purpose

   Level 1 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJUMP27-1A(sfGFP)

   Plasmid

   #126974

   Purpose

   Level 1 vector. sfGFP reporter. Origin pSC101 (low copy number)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJUMP47-2A(sfGFP)

   Plasmid

   #126991

   Purpose

   Level 2 vector. sfGFP reporter. Origin pSC101 (low copy number)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWPXL-c-Myc

   Plasmid

   #36980

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   c-Myc (Myc Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

8. SGK2 gRNA (BRDN0001149504)

   Plasmid

   #76208

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SGK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SGK2 (SGK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. SGK2 gRNA (BRDN0001147898)

   Plasmid

   #76209

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SGK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SGK2 (SGK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. SGK2 gRNA (BRDN0001145816)

    Plasmid

    #76206

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SGK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SGK2 (SGK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. SGK2 gRNA (BRDN0001148976)

    Plasmid

    #76207

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SGK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SGK2 (SGK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-ERT2CreERT2

    Plasmid

    #13777

    Purpose

    Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    ERT2-Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-CreERT2

    Plasmid

    #14797

    Purpose

    Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJUMP27ts-1A(sfGFP)

    Plasmid

    #126975

    Purpose

    Level 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    RepA A56V thermosensitive mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX458-HypaCas9

    Plasmid

    #190604

    Purpose

    PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSKI

    Plasmid

    #232484

    Purpose

    pSKI can be used for single-copy transgene expression within the Universal SKI System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.

    Depositor

    Carlos Silva García

    Article

    Dinneen et al bioRxiv [Preprint]. 2024 Dec 10:2024.12.

    Insert

    pSKI
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. p663-UBC-miniTurbo-V5-TP53RK_IDG-K

    Plasmid

    #170589

    Purpose

    Gateway destination clone of TP53RK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-TP53RK (TP53RK Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-pgk-DIO-rvCRY-WPRE

    Plasmid

    #182060

    Purpose

    Plasmids for production of AAV-based TFactivity reporter

    Depositor

    Kentaro Abe

    Article

    Abe et al iScience. 2022 Feb 14;25(3):103927. doi:

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hu Pgk1

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJUMP47ts-2A(sfGFP)

    Plasmid

    #126992

    Purpose

    Level 2 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    RepA A56V thermosensitive mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXJ42-p200 CUX1

    Plasmid

    #100813

    Purpose

    Plasmid vector expressing human p200 CUX1 (amino acids 1-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag

    Depositor

    Alain Nepveu

    Article

    Wilson et al J Biol Chem. 2009 Jan 2;284(1):36-45. do

    Insert

    p200 CUX1 (CUX1 Human)
    

    Use

    Note that this plasmid does not have an sv40 ori

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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