We narrowed to 3,128 results for: try
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Plasmid#232200PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreenDepositorInsertmNeongreen
ExpressionBacterialAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Human
Plasmid#238130PurposeThis plasmid encodes the full-length human TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.DepositorInsertTMEM106B (TMEM106B Human)
UseLentiviralTagsBlasticidin-P2A-3xHAExpressionMammalianPromoterCMVAvailable sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJCC_075 AsCas12a D908A
Plasmid#171668PurposeFor bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a D908A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 908 to alanineAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJCC_076 AsCas12a R1226A
Plasmid#171669PurposeFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a R1226A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginine 1226 to alanineAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-eDHFR-Cdc42Q61L
Plasmid#107267PurposemCherry-eDHFR-Cdc42Q61L in pIVT vector for in vitro transcription. Note that Cdc42 in this plasmid keeps its CAAX domain.DepositorAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-iSpyMac
Plasmid#149553PurposeGateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMsDepositorInsertiSpyMacCas9
UseCRISPR; Gateway compatible zispymaccas9 entry clo…Tags3X FLAG, NLS and NLSExpressionPlantMutationR221K, N394K, Protospacer interacting domain form…Available sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
phpRL-BCL2-FL-pA
Plasmid#42595DepositorInserthairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase
UseLuciferaseExpressionMammalianPromoterCMVAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RGG-BcLOV-mCh-EGFR
Plasmid#208280PurposeExpresses RGG-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.DepositorInsertRGG-BcLOV-mCh-EGFR
UseLentiviralMutationH225N in mCherry- please see depositor commentPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 W47A-mycDDK
Plasmid#137769PurposeCALCOCO1 LIR mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCALCOCO1 W47A
TagsmycDDKExpressionMammalianMutationW47APromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx50-20xMS2-Hyg
Plasmid#210219PurposeIntegrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
Available sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC-N-GFP
Plasmid#172974PurposeMAC-tagged GFP expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP
TagsMAC-tagExpressionBacterial and MammalianAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 Orf8
Plasmid#158377PurposeMAC-tagged gene expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 Orf8 (ORF8 )
TagsMAC-tagExpressionMammalianAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-SARS-CoV-2 Orf3a
Plasmid#158372PurposeMAC-tagged gene expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 Orf3a (ORF3a )
TagsMAC-tagExpressionMammalianAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-SARS-CoV-2 NSP11
Plasmid#158362PurposeMAC-tagged gene expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 NSP11 (ORF1ab )
TagsMAC-tagExpressionMammalianAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-SARS-CoV-2 NSP12
Plasmid#158363PurposeMAC-tagged gene expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 NSP12 (ORF1ab )
TagsMAC-tagExpressionMammalianAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p3E-EGFP-NS-MCS
Plasmid#75180PurposeEGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-NS-MCS
UseThree prime entry vector for multisite gateway th…Promotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEXndoS(E235Q)
Plasmid#44656DepositorInsertndoS (ndoS Streptococcus pyogenes)
TagsGstExpressionBacterialMutationChanged Glutamic acid 235 to GlutaminePromotertacAvailable sinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMMS22L-siR3
Plasmid#221013Purposemammalian expression vector of myc-Flag tagged MMS22L siRNA resistantDepositorInsertMMS22L (MMS22L Human)
TagsFlag and mycExpressionMammalianMutationsilent mutations MMS22L: 5'-aaAacGtgTtgCtgTg…PromoterCMVAvailable sinceJuly 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBad-CA TAG20
Plasmid#105666Purposeexpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tagDepositorPublicationInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialMutationTAG stop codon at site 20PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
mVenus-IQ6 WT
Plasmid#198201PurposeFRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMYO5A IQ domain (Myo5a Mouse)
TagsmVenusExpressionMammalianAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only