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pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9

Plasmid

#163550

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-HF-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 249 (pTol2-Dll4-F2-ETS#2-WT-8X-E1b-EGFP)

Plasmid

#156417

Purpose

8X copies of the ETS#2 site of the murine Dll4 intronic enhancer and a minimal E1b reporter driving expression of EGFP flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

murine Dll4 F2-6/F8 ETS site B/ site #2, 8X

Use

Zebrafish transgenesis

Promoter

E1b min pro/b-globin intron

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-DDX43-NT-G46D

Plasmid

#134588

Purpose

Express human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)

Tags

His

Expression

Bacterial

Mutation

full-length (G154D)

Promoter

T7

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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MoFGFR31c-ires neo

Plasmid

#86496

Purpose

mamalian cell expression of FGFR3-FGFR1 chimera

Depositor

David Ornitz

Article

Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

Insert

FgfrR3-1 IIIc 15C7 (Fgfr1, Fgfr3 Mouse)

Expression

Mammalian

Mutation

FGFR3-FGFR1 chimera

Promoter

Moloney murine leukemia virus LTR

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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MoFGFR31b-ires neo

Plasmid

#86495

Purpose

mamalian cell expression of FGFR3-FGFR1 chimera

Depositor

David Ornitz

Article

Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

Insert

FgfrR31 IIIb 15C8 (Fgfr3, Fgfr1 Mouse)

Expression

Mammalian

Mutation

FGFR3-FGFR1 chimera

Promoter

Moloney murine leukemia virus LTR

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSico shMAP3K2 mouse

Plasmid

#85657

Purpose

expression of shRNA targeting mouse MAP3K2

Depositor

Julien Sage

Article

Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

Insert

shRNA targeting 3'UTR of Map3k2 (Map3k2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSico shMAP3K2 human

Plasmid

#85656

Purpose

expression of shRNA targeting human MAP3K2

Depositor

Julien Sage

Article

Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

Insert

shRNA targeting 3'UTR of MAP3K2 (MAP3K2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMiR-CIP2A-miR-375-mutC&D

Plasmid

#53712

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site C&D (refer t…

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pBullet-er-n

Plasmid

#53067

Purpose

destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-tgn-n

Plasmid

#53071

Purpose

destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pMiR-CIP2A-miR-375-mutE

Plasmid

#53710

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site E (refer to cita…

Available Since

July 11, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutA&B

Plasmid

#53711

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site A&B (refer t…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutD

Plasmid

#53709

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site D (refer to cita…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutC

Plasmid

#53708

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site C (refer to cita…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutB

Plasmid

#53707

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site B (refer to cita…

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutA

Plasmid

#53706

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site A (refer to cita…

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pBIG2abc_hsHAUScomplex_FL

Plasmid

#202203

Purpose

Baculoviral transfer vector to co-express 8 subunits of human augmin complex

Depositor

Thomas Surrey

Article

Ustinova et al J Cell Sci. 2023 Jun 15;136(12):jcs26109

Inserts

HAUS1 (HAUS1 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS3 (HAUS3 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS2 (HAUS2 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS6 (HAUS6 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS7 (HAUS7 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS4 (HAUS4 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS5 (HAUS5 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS8 (HAUS8 codon-optimized form for expression in Trichoplusia ni cells, Human)

Tags

-mGFP-TEV-TwinStrep and His-tag (6x)

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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Syn61Δ3(ev5)

Bacterial Strain

#174514

Purpose

Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Bacterial Resistance

Streptomycin

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only
ACBE-Q

Plasmid

#204608

Purpose

Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

Insert

bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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ACBE

Plasmid

#204607

Purpose

Mammalian expression of adenine-to-cytosine base editor ACBE

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

Insert

bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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