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pRSET FLIPmal

Plasmid

#14478

Depositor

Wolf Frommer

Article

Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

Insert

FLIPmal

Tags

ECFP and EYFP

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pCDEF3‐mTim1 (no tag)

Plasmid

#49206

Purpose

expresses mouse Tim1

Depositor

Lawrence Kane

Article

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (Havcr1 Mouse)

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-BEC enhancer-minBG-SYFP2

Plasmid

#236722

Purpose

SYFP2-tagged Brain Endothelial Cell Enhancer mini Beta Goblin promoter

Depositor

Xiangmin Xu

Article

Velazquez-Rivera et al Neuron. 2025 May 21;113(10):1562-1578.e6

Type

Empty backbone

Use

AAV

Tags

SYFP2

Promoter

minimal beta globulin

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-CR029

Plasmid

#234041

Purpose

expresses evoCDA1 BE4max base editor in a human T cell optimized lentiviral transfer plasmid

Depositor

Alexander Marson

Article

Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

Insert

evoCDA1-Cas9(D10A)-P2A-blast

Use

Lentiviral

Tags

blast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA Donor_hEF1a

Plasmid

#229779

Purpose

Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pC1_oROS-HT

Plasmid

#216413

Purpose

Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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CROPseq-Guide-BSD

Plasmid

#216123

Purpose

Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-602_NY-ESO-1_TCR_RFP

Plasmid

#207483

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP, NY-ESO-1_TCR

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-fullef1a-FAKSPARK2

Plasmid

#195265

Purpose

express wtFAKSPARK2 in a lenti vector

Depositor

Xiaokun Shu

Article

Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

Insert

FAKSPARK2 sensor

Use

Lentiviral

Expression

Mammalian

Promoter

ef1a

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_ONNV_E1E2

Plasmid

#207296

Purpose

Mammalian expression of Onyong-nyong virus E1E2 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

ONNV_E1E2

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-176

Plasmid

#195424

Purpose

Transient, constitutive expression of synZiFTR: ZF1-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF1-3 (ZF1) - p65 - RbGpA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-178

Plasmid

#195426

Purpose

Transient, constitutive expression of synZiFTR: ZF3-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF3-5 (ZF3) - p65 - RbGpA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-185

Plasmid

#195433

Purpose

Transient, constitutive expression of synZiFTR: ZF10-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF10-1 (ZF10) - p65 - RbGpA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR

Plasmid

#192636

Purpose

Expresses ERT2-CreN59-nMag-NLS with mCherry marker.

Depositor

Yingxiao Wang

Article

Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

Insert

ERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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EK0396 BFPmut-stop-t70587 (pEAQ)

Plasmid

#191171

Purpose

EK0208 in plant-expressable format; Trigger 1 in 3' UTR of an inactive BFP

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

t70587 (plant)

Expression

Plant

Mutation

WT

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-B3

Plasmid

#191668

Purpose

ddFP-B3-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Tags

HA and ddFP-B3

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

Plasmid

#167902

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->Gss52SR4-LaM4-P2A-NLSIFP

Plasmid

#162229

Purpose

Lentiviral vector for the expression of Gss52SR4-LaM4-P2A-NLSIFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG17 GFP nanobody SynNotch tTA

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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IDG_CACNG3_OE_1

Plasmid

#161622

Purpose

Overexpresses CACNG3

Depositor

Mike McManus

Insert

CACNG3 (CACNG3 Human)

Tags

V5

Expression

Mammalian

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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IDG_CACNG8_OE_1

Plasmid

#161698

Purpose

Overexpresses CACNG8

Depositor

Mike McManus

Insert

CACNG8 (CACNG8 Human)

Tags

V5

Expression

Mammalian

Available Since

Dec. 22, 2021

Availability

Academic Institutions and Nonprofits only

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