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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,971 results for: nin

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Showing: 10621 - 10640 of 66971 results

  4. mCh-KIF13A*-strep

    Plasmid
    #120166
    Purpose
    Expresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidin
    Depositor
    Juan Bonifacino
    Publication
    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    kinesin family member 13A (KIF13A Human)
    Tags
    Streptavidin and mCherry
    Expression
    Mammalian
    Mutation
    Truncated KIF13A: 1-639 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120166-plasmid-map-sequence-id-247501

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  5. EHD2-T72A-mEGFP

    Plasmid
    #45934
    Purpose
    dominant-negative EHD2 mutant
    Depositor
    Ari Helenius
    Publication
    Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Eps-15 homology domain-containing protein2 (EHD2 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    T 72 changed to A (T72A)
    Promoter
    CMV
    Available since
    Aug. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    45934-plasmid-map-sequence-id-66948

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  6. pCAG-AILR-LplA-ER

    Plasmid
    #42574
    Depositor
    Alice Ting
    Publication
    Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LplA(AILR) with ER-retention sequence KDEL
    Tags
    FLAG
    Mutation
    Compared to wild-type LplA: tryptophan 37 to alan…
    Promoter
    CAG
    Available since
    April 11, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. SPACE-ΔUGI (pRZ1836)

    Plasmid
    #140243
    Purpose
    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SPACE-ΔUGI
    Expression
    Mammalian
    Mutation
    V82G in TadA*, D10A in Cas9, R187W in pmCDA1
    Promoter
    CMV
    Available since
    June 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    140243-plasmid-map-sequence-id-275726

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  8. H52-pUCM-hNGN2-inv

    Plasmid
    #110492
    Purpose
    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm
    Depositor
    Michael Ward
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    Use
    CRISPR, Cre/Lox, and TALEN
    Expression
    Mammalian
    Promoter
    CAG, EF-1 alpha, and TRE3G
    Available since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    110492-plasmid-map-sequence-id-207872

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  9. GIV-F1685A FLAG

    Plasmid
    #65948
    Purpose
    Expression GIV/Girdin F1685A mutation in Mammalian cell
    Depositor
    Pradipta Ghosh
    Publication
    Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GIV/Girdin (CCDC88A Human)
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Phenylalanine 1685 to Alanine
    Promoter
    CMV
    Available since
    Jan. 7, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pAB1923 REPAIR.t1-S

    Plasmid
    #176321
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q/E620G/Q696L, only the deaminase domain (aa …
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176321-plasmid-map-sequence-id-345288

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  15. EBA181-bio

    Plasmid
    #47744
    Purpose
    Expresses enzymatically monobiotinylated full-length EBA181 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon-optimised EBA181
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47744-plasmid-map-sequence-id-68752

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  20. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

    Plasmid
    #197508
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…
    Promoter
    CMV and T7
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197508-plasmid-map-sequence-id-387292

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Showing: 10621 - 10640 of 66971 results