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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,358 results for: grn

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Showing: 10701 - 10720 of 17358 results

  3. pIF1079

    Plasmid
    #237445
    Purpose
    Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.
    Depositor
    IIya Finkelstein
    Article
    Buffington et al Nat Biotechnol. 2025 Oct 23. doi: 10.103
    Inserts
    SpCas9
    Efe1-RT
    EMX1 sgRNA
    Efe1-ncRNA targeting EMX1
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CAG, H1, and U6
    Available Since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    237445-plasmid-map-sequence-id-476973

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  6. LentiGuide Cherry

    Plasmid
    #170510
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Pavel Tolar
    Article
    Newman et al bioRxiv 2021.03.02.433398
    Inserts
    S. pyogenes sgRNA cassette
    mCherry
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170510-plasmid-map-sequence-id-343694

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  9. pYPQAT57

    Plasmid
    #223429
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223429-plasmid-map-sequence-id-453502

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  11. pYPQAT58

    Plasmid
    #223430
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223430-plasmid-map-sequence-id-454181

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  12. pdCas9

    Plasmid
    #46569
    Purpose
    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9
    CRISPR array
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46569-plasmid-map-sequence-id-364492

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  16. pCBC-MT1T2

    Plasmid
    #50593
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50593-plasmid-map-sequence-id-451910

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  17. pRCC-K

    Plasmid
    #81191
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81191-plasmid-map-sequence-id-154417

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Showing: 10701 - 10720 of 17358 results