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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,278 results for: nes

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Showing: 10741 - 10760 of 16278 results

  1. PB-PE NG

    Plasmid
    #174554
    Purpose
    All-in-one prime editor piggyBac transposon, NG variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    L1111R, G1218R, E1219F, A1322R, R1335V, T1337R
    Promoter
    CAG
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    174554-plasmid-map-sequence-id-354966

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  2. p1.2-Zeo-eGFP

    Plasmid
    #162743
    Purpose
    Fluorescent reporter for CHO cells expression studies
    Depositor
    Ivan Vorobiev
    Publication
    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enhanced green fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162743-plasmid-map-sequence-id-339096

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  3. Mouse 3' Ubf AID GFP PuroR

    Plasmid
    #127901
    Purpose
    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Ubf Gene
    Depositor
    Mark Groudine
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ubf (Ubtf Mouse, Mustard Weed)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Mutation
    Inserting GFP AID 2A Puro into mouse 3' Ubf
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127901-plasmid-map-sequence-id-249684

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  5. mutpRL-TK 3xUTR

    Plasmid
    #40758
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. pAAV-hsyn-DIO-AppNL-G-F-T2A-mCherry

    Plasmid
    #242649
    Purpose
    Floxed (DIO) AAV transgene designed to be packaged in AAV and to express the amyloid precursor protein (App) with familial mutations AppNL-G-F in a Cre-recombinase-dependent manner.
    Depositor
    William Wisden
    Insert
    Floxed (DOI) AAV transgene expressing an amyloid precursor protein with familial mutations and an mCherry tag
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Expression
    Mammalian
    Mutation
    The amyloid precursor reading frame contains a c…
    Promoter
    human synapsin
    Available since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    242649-plasmid-map-sequence-id-489352

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  10. pT2iU6m5Hoxa13bE4PCh(#765)

    Plasmid
    #184102
    Purpose
    cyclofen-inducible fish Hoxa13b activation via zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    5myc-ZfHoxa13b-ERT2-P2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes) + in vitro tra…
    Tags
    5xMyc
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    Zf-Ubi+SP6
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184102-plasmid-map-sequence-id-365950

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  11. pT22cUm5En2E4P3Che(#1188)

    Plasmid
    #184094
    Purpose
    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    5myc-chkEn2-ERT2-5'fP2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    5xMyc
    Mutation
    5'fP2A: GSG-P2A; ERT2: G400V, M543A, L544A, …
    Promoter
    Zf-Ubi
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184094-plasmid-map-sequence-id-363967

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  13. Epac-S-H150

    Plasmid
    #170345
    Purpose
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170345-plasmid-map-sequence-id-344646

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  14. Epac-S-H94

    Plasmid
    #170341
    Purpose
    GFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP(UST)_EPACdDEPCD_mCherry (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170341-plasmid-map-sequence-id-335921

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  15. pKIF1C-2xFLAG

    Plasmid
    #130979
    Purpose
    Expression of KIF1C-2xFLAG in mammalian cells.
    Depositor
    Anne Straube
    Publication
    Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIF1C-2xFLAG (KIF1C Human)
    Tags
    2xFLAG
    Expression
    Mammalian
    Mutation
    4 silent mutations that make this construct resis…
    Promoter
    CMV
    Available since
    Oct. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    130979-plasmid-map-sequence-id-259357

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  16. pVZ_PcoaT_SQE1_LUP1

    Plasmid
    #103857
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and LUP1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Publication
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    lupeol synthase 1 (LUP1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    103857-plasmid-map-sequence-id-217123

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  17. GFP-KIF1B beta-600-1400

    Plasmid
    #79109
    Purpose
    Mammalian expression of GFP-KIF1B beta-600-1400
    Depositor
    Susanne Schlisio
    Publication
    Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP-KIF1B beta (600-1400 amino acids) (KIF1B Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    exon 25 missing (natural splice varient)
    Promoter
    CMV
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pcDNA3-mCherry-TSC2(AA)-NLS

    Plasmid
    #192443
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192443-plasmid-map-sequence-id-379076

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Showing: 10741 - 10760 of 16278 results