We narrowed to 7,375 results for: ef1a

1. Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP

   Plasmid

   #157951

   Purpose

   Lentiviral expression of HBG site 1 sgRNA

   Depositor

   Dali Li

   Article

   Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHIE058 ZF43x0 mKate2 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138739

   Purpose

   Integration Vector, assembled with pHIE042 (TUPV1), pJM451 (TUPV2), pPD612

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x0
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE

   Plasmid

   #118279

   Purpose

   Expresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cells

   Depositor

   Dietmar Schmitz

   Article

   Wozny et al Front Cell Neurosci. 2018 Oct 2;12:337.

   Inserts

   mRuby2
   ChR2(H134R)
   

   Use

   AAV and Cre/Lox

   Tags

   SV40 NLS and YFP

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin

   Plasmid

   #134866

   Purpose

   Expresses EGFP-tagged mutant of VAMP1

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Vesicle-associated membrane protein 1 (VAMP1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 97-118

   Promoter

   EF1a

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Dmap1(SDM)

   Plasmid

   #117139

   Purpose

   Expression vector encoding BFP-BSD-HA-Dmap1 resistant against gRNA encoded by pKLV2-U6(gDmap1 v4)--PGK-Puro-BFP

   Depositor

   Kosuke Yusa

   Article

   Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

   Insert

   HA-Dmap1 (mutagenised) (Dmap1 Synthetic)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   modified BFP, modified Dmap1 CDS

   Promoter

   human U6 promoter, human EF1a promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75243

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Ikaros (IKZF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75234

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc2 (NFATC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75235

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc2 (NFATC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75242

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Ikaros (IKZF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75238

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75239

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EF1a-ZIM3-Cas9-P2A-GFP-PGK-Blasti

    Plasmid

    #239610

    Purpose

    Constitutive active CRISPRgenee construct with a blasticidin resistance

    Depositor

    Philipp Rathert

    Article

    Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

    Insert

    ZIM3 KRAB domain (ZIM3 )
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EF1a-FLEX-Gephyrin.FR-GFP-CCR5TC-p2A-mRuby

    Plasmid

    #245309

    Purpose

    Expression of synaptic GFP and cytosolic mRuby

    Depositor

    Xue Han

    Insert

    Gephyrin.FR-GFP-CCR5TC-p2A-mRuby
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EF1a-FLEX-mRuby-p2A-Gephyrin.FR-GFP-CCR5TC

    Plasmid

    #245308

    Purpose

    Expression of synaptic GFP and cytosolic mRuby

    Depositor

    Xue Han

    Insert

    mRuby-p2A-Gephyrin.FR-GFP-CCR5TC
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgRNA-Dual_filler(hU6_H1)-EF1a-Thy1.1-P2A-Neo

    Plasmid

    #239608

    Purpose

    Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and H1 promoters) using BsmbI. Selectable with a G418 resistance

    Depositor

    Philipp Rathert

    Article

    Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

    Insert

    filler-trcr-H1-filler
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV EF1alpha INTS4 K26/27A IRES Cherry

    Plasmid

    #228111

    Purpose

    Lentiviral expression of human INTS4 K26/27A

    Depositor

    Tom Misteli

    Article

    Sood et al Mol Cell. 2025 Mar 6;85(5):913-928.e8. d

    Insert

    INTS4 (INTS4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mutated Lysine 26 and 27 to Alanine

    Promoter

    EF 1alpha

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV EF1alpha INTS4 K26/27Q IRES Cherry

    Plasmid

    #228112

    Purpose

    Lentiviral expression of human INTS4 K26/27Q

    Depositor

    Tom Misteli

    Article

    Sood et al Mol Cell. 2025 Mar 6;85(5):913-928.e8. d

    Insert

    INTS4 (INTS4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mutated Lysine 26 and 27 to Glutamine

    Promoter

    EF 1alpha

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-EF1A-TRIP12-391-938-EGFP-Hygro

    Plasmid

    #238092

    Purpose

    Expression of structurally predicted WWE domain of TRIP12 with EGFP on C terminal

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    TRIP12 (TRIP12 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 319-938 only

    Promoter

    EF1alpha

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-Hygro-EF1A-TRIP12(749-836)-EGFP

    Plasmid

    #196229

    Purpose

    EGFP fused to the C-terminus of a WWE domain within TRIP12 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    C-terminal EGFP tagged TRIP12(749- 836)
    

    Use

    Lentiviral

    Tags

    EGFP and Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-Hygro-EF1A-Af1521(K35E/Y145R)-EGFP

    Plasmid

    #196235

    Purpose

    EGFP fused to the C-terminus of Af1521(K35E/Y145R) macrodomain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    C-terminal EGFP tagged Af1521(K35E/Y145R)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K35E and Y145R

    Promoter

    EF1A

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV-Hygro-EF1A-RNF146(100-182)-myc

    Plasmid

    #196236

    Purpose

    RNF146(100-182) WWE domain with a myc tag fused to the C terminus & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    RNF146(100-182) encoding a C-terminal myc tag
    

    Use

    Lentiviral

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV-Hygro-EF1A-Af1521(K35E/Y145R)-myc

    Plasmid

    #196237

    Purpose

    Af1521(K35E/Y145R) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    Af1521(K35E/Y145R) encoding a C-terminal myc tag
    

    Use

    Lentiviral

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    amino acid 35 lysine is replaced with glutamic ac…

    Promoter

    EF1A

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. (942) pAAV EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid

    #195624

    Purpose

    pAAV EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct

    Depositor

    Tonia Rex

    Insert

    dCasphi
    

    Use

    AAV

    Tags

    C-Myc (NLS), HA tag, and ZIM3

    Expression

    Mammalian

    Mutation

    P749A and P753A

    Promoter

    EF1a (Mlu1/EcoR1)

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH

    Plasmid

    #235301

    Purpose

    ComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    FXN-P2A-mRuby2 (FXN Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pL(EF1a-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)

    Plasmid

    #236279

    Purpose

    Lentiviral vector that can express hAqp1 with fkbp12 degron in mammalian cells. EF1a promoter. EGFP marker.

    Depositor

    Arnab Mukherjee

    Article

    Yun et al Chem Sci. 2024 Jun 4;15(28):11108-11121.

    Insert

    human aquaporin 1 (AQP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag and fkbp12

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH

    Plasmid

    #235302

    Purpose

    ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    FXN-P2A-mRuby2 (FXN Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-Ef1a-Con/Fon-Synaptophysin-10xMyc-WPRE

    Plasmid

    #237446

    Purpose

    Intersectional expression of Myc-tagged synaptophysin in the presence of Cre and Flp

    Depositor

    Silvia Arber

    Insert

    Con/Fon-Synaptophysin-10xMyc
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

    Plasmid

    #233442

    Purpose

    PiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR)

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. XZ362 EF1a-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export

    Plasmid

    #233381

    Purpose

    Optimized VEGF-sensing eLIDAR with VEGFR2 ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequence

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    VEGFR2(ecto+TM)-tdMCP
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1a

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-EF1a-mHFE(H67D)-IRES-GFP-WPRE-pA

    Plasmid

    #233040

    Purpose

    To express mouse homeostatic iron regulator (HFE)(H67D) from an EF1a promoter. It also encodes GFP after an IRES sequence

    Depositor

    Jonathan Ploski

    Insert

    mouse homeostatic iron regulator with H67D mutation (Hfe Mouse)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mutation

    H67D

    Promoter

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge