Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 4,214 results for: bre

Showing: 2651 - 2700 of 4214 results

  3. pLKO.1-shGBP1.1.mKO2

    Plasmid
    #85209
    Purpose
    TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.
    Depositor
    Sandra Martha Gomes Dias
    Article
    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1
    Insert
    GBP1 (guanylate binding protein 1) (GBP1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA fo…
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85209-plasmid-map-sequence-id-169780

    Enlarge

  15. dCas9-NLS-gs10-cDHFR174

    Plasmid
    #188256
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split DHFR (aa 1-173), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cDHFR174 (DHFR Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188256-plasmid-map-sequence-id-374594

    Enlarge

  34. pCVL.SSA TLR (CCR5 TALEN Sce Spacer)

    Plasmid
    #46941
    Purpose
    Codes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backbone
    Depositor
    Andrew Scharenberg
    Article
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Inserts
    5' iRFP arm
    eGFP with TALEN TS
    +3 mCherry
    3' iRFP arm
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    embedded TALEN TS from 163-218, truncated 25 amin…
    Promoter
    SFFV
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    46941-plasmid-map-sequence-id-67512

    Enlarge

  35. pBrain-GFP-TACC3KDP(S558A)-shTACC3

    Plasmid
    #59357
    Purpose
    Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.
    Depositor
    Stephen Royle
    Article
    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10
    Insert
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    Use
    RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Silent mutations in amino acids 30-33 to confer s…
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    59357-plasmid-map-sequence-id-94887

    Enlarge

  40. AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

    Plasmid
    #120294
    Purpose
    AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)
    Depositor
    Dominik Niopek
    Article
    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545
    Insert
    C-terminal fragment of SpyCas9
    Use
    AAV and CRISPR
    Tags
    split-intein
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    120294-plasmid-map-sequence-id-232735

    Enlarge

Showing: 2651 - 2700 of 4214 results