We narrowed to 5,789 results for: aaas

1. pLYS6-METTL17-LYRMut-FLAG

   Plasmid

   #220877

   Purpose

   Expressing METTL17-LYRMut-FLAG

   Depositor

   Vamsi Mootha

   Article

   Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

   Insert

   METTL17 (METTL17 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed the LYP domain to AAA

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. psicheck2-MAIP1mut

   Plasmid

   #203604

   Purpose

   Reporter plasmid carrying MAIP1 3'UTR with mutated miR27b target sequences

   Depositor

   Hiroyuki Mizuguchi

   Article

   Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

   Insert

   matrix AAA peptidase interacting protein 1 (MAIP1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   miR-27b target sequences in the 3'UTR was mu…

   Promoter

   SV40

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. QPM-sgR (pTaU3)

   Plasmid

   #140448

   Purpose

   For sgRNA expression in monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

   Insert

   TaU3-sgRNA
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   TaU3

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. L1_lacZgRNA-Ck3

   Plasmid

   #136136

   Purpose

   L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpU6:lacZgRNA
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. L1_lacZgRNA-Ck2

   Plasmid

   #136137

   Purpose

   L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpU6:lacZgRNA
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJ23119-sgRNA

   Plasmid

   #113654

   Purpose

   sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

   Depositor

   Jeffrey Barrick

   Article

   Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

   Insert

   promoter J23119 and sgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. ADEPT-pTarget-GFP

   Plasmid

   #238041

   Purpose

   Encodes sfGFP under lac promoter. Expresses a single guide RNA (under Rha promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.

   Depositor

   Lingchong You

   Article

   Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Promoter

   lac

   Available Since

   June 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. ADEPT-pTarget-RFP

   Plasmid

   #238040

   Purpose

   Encodes mRFP1 under pLux promoter. Expresses a single guide RNA (under Ara promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.

   Depositor

   Lingchong You

   Article

   Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Promoter

   Lux

   Available Since

   June 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210734

   Purpose

   Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Frodo sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR-cBEST

    Plasmid

    #125689

    Purpose

    C to T base editor for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

    Insert

    streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    ermE*/tipA

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDY2259 hNOLC1 atgRNA Paired Guide 1

    Plasmid

    #220989

    Purpose

    hNOLC1 atgRNA

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

    Insert

    NOLC1 atgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDY2260 hNOLC1 atgRNA Paired Guide 2

    Plasmid

    #220990

    Purpose

    hNOLC1 atgRNA

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

    Insert

    NOLC1 atgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDY2261 hACTB atgRNA Paired Guide 1

    Plasmid

    #220991

    Purpose

    hACTB atgRNA

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

    Insert

    ACTB atgRNA (ACTB Human)
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDY2262 hACTB atgRNA Paired Guide 2

    Plasmid

    #220992

    Purpose

    hACTB atgRNA

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

    Insert

    ACTB atgRNA (ACTB Human)
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-sgE1TSS-short-3'box

    Plasmid

    #92165

    Purpose

    sgRNA expression vector for Evx1as RNA tethering assay

    Depositor

    Xiaohua Shen

    Article

    Luo et al Cell Stem Cell. 2016 May 5;18(5):637-52.

    Insert

    Evx1as short isoform
    

    Use

    CRISPR

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWT055g

    Plasmid

    #96864

    Purpose

    sgRNA-HEK3

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    sgRNA-HEK3
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWT055e

    Plasmid

    #96866

    Purpose

    sgRNA-EMX1

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    sgRNA-EMX1
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWT055h

    Plasmid

    #96867

    Purpose

    sgRNA-HEK4

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    sgRNA-HEK4
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWT055f

    Plasmid

    #96865

    Purpose

    sgRNA-FANCF

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    sgRNA-FANCF
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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