We narrowed to 17,121 results for: bon

1. pMDC123SB-AtTAS1c-B/c

   Plasmid

   #51772

   Purpose

   Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

   Depositor

   James Carrington

   Article

   Carbonell et al Plant Physiol. 2014 Mar 19.

   Insert

   AtTAS1c-B/c
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   A. thaliana TAS1c precursor sequence including a …

   Promoter

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHJ4: LexA-VHH-Caffeine

   Plasmid

   #108242

   Purpose

   LexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.

   Depositor

   Jerome Bonnet

   Article

   Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

   Insert

   LexA DNA binding domain fusing with VHH-Caffeine antibody
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pLacO1

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)

   Plasmid

   #134333

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA17_Efa
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI-neo VPS50-13Myc

   Plasmid

   #175093

   Purpose

   13Myc tagged VPS50 is expressed in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Schindler et al Nat Cell Biol. 2015 May;17(5):639-50. do

   Insert

   VPS50 (CCDC132 Human)
   

   Use

   Tags

   13Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTripz Casp1-IRES-mCherry

   Plasmid

   #187294

   Purpose

   This is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).

   Depositor

   Alpha Yap

   Article

   Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

   Insert

   Caspase1 (Casp1 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA

   Plasmid

   #51473

   Purpose

   Bicistronic vector expressing mRuby2 and GCaMP6m from a single open reading frame.

   Depositor

   Mark Huebener, Tobias Rose, Tobias Bonhoeffer

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Insert

   mRuby2-P2A-GCaMP6m
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Str-STIM1-NN_ST-SBP-mCherry

   Plasmid

   #65263

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-4T-1-β4 hinge-ear

   Plasmid

   #196692

   Purpose

   Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

   Insert

   AP-4 β4 hinge-ear (534-739) (AP4B1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-AtTAS1c-B/c

   Plasmid

   #51774

   Purpose

   Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

   Depositor

   James Carrington

   Article

   Carbonell et al Plant Physiol. 2014 Mar 19.

   Insert

   AtTAS1c-B/c
   

   Use

   Gateway-compatible entry clone

   Tags

   Expression

   Mutation

   A. thaliana TAS1c precursor sequence including a …

   Promoter

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. TIRAP-GFP

    Plasmid

    #52739

    Purpose

    Retroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable marker

    Depositor

    Jonathan Kagan

    Article

    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

    Insert

    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pAAV_Flex_syn_SpikeyGi2_kv2.1

    Plasmid

    #195314

    Purpose

    Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, double-flowed in AAV vector under the promoter hSyn

    Depositor

    Vincent Pieribone

    Article

    Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

    Insert

    SpikeyGi2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF6 VPS51-V5-6His

    Plasmid

    #175502

    Purpose

    V5 tagged VPS51 is expressed in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Perez-Victoria et al Mol Biol Cell. 2010 Oct 1;21(19):3386-95

    Insert

    VPS51 (VPS51 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    A661V- please see depositor comments

    Promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBeast-pAhpC-RBS- LacZ

    Plasmid

    #190060

    Purpose

    LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    lacZ
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBeast-pT7-soxA

    Plasmid

    #190057

    Purpose

    Express the bacterial monomeric sarcosine oxidase enzyme under the control of the strong constitutif phage promoter T7

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    SoxA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DMP1-GFPtopaz

    Plasmid

    #114270

    Purpose

    GFP labeling of mature osteoblasts and osteocytes.

    Depositor

    David Rowe

    Article

    Kalajzic et al Bone. 2004 Jul;35(1):74-82. doi: 10.1016

    Insert

    Dmp1 + GFPtopaz (Dmp1 Aequorea victoria, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dmp1

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDF11-SFPQ(276-598)

    Plasmid

    #135437

    Purpose

    Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    Splicing Factor Proline And Glutamine Rich (SFPQ Human)
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pH7WG2B-OsMIR390-B/c

    Plasmid

    #61465

    Purpose

    Plant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

    Depositor

    James Carrington

    Article

    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

    Insert

    OsMIR390-B/c
    

    Use

    Tags

    Expression

    Plant

    Mutation

    O. sativa MIR390 precursor sequence including a c…

    Promoter

    OsUBI

    Available since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFE-hCA

    Plasmid

    #132999

    Purpose

    pFE carrying a human carbonic anhydrase II gene (Uniprot: P00918)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Insert

    hCAII (CA2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetR

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBeast-pAhpC-RBS- sfGFP

    Plasmid

    #190051

    Purpose

    GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

    Plasmid

    #115137

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS

    Depositor

    Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA2.1 anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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