We narrowed to 23,671 results for: CRISPR

1. AA331

   Plasmid

   #215954

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v2 [Sa]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJJGL002

   Plasmid

   #180606

   Purpose

   Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Insert

   PlmCasX
   

   Use

   CRISPR

   Tags

   10xHis and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCK340

   Plasmid

   #192639

   Purpose

   Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Inserts

   Sp.pCas9-dSpG
   BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   SoxS has R93A and S101A mutations and dSpG has se…

   Promoter

   BBa_J23107 and Sp.pCas9

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57-white[coffee]

   Plasmid

   #84006

   Purpose

   Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4

   Depositor

   Phillip Zamore

   Article

   Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

   Insert

   A 2,080 bp fragment of the white[coffee] allele (w Fly)
   

   Use

   Unspecified

   Mutation

   A GC-to-AA mutation that creates a G589E missense…

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. BE3-P2A-EGFP (pJUL977)

   Plasmid

   #123612

   Purpose

   CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ7751 pHR Ef1α: mCherry-P2A-AcrIIA4

   Plasmid

   #125148

   Purpose

   Lentiviral vector for constitutive expression of AcrIIA4 with mCherry fluorescent reporter.

   Depositor

   Stanley Qi

   Article

   Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

   Insert

   AcrIIA4-P2A-mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ133-RZ-Cas12j2

   Plasmid

   #189784

   Purpose

   Golden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-GG-hUBC-EBFP2

   Plasmid

   #212314

   Purpose

   Lentiviral backbone with EBFP2 reporter

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNOC_hlbCas12a-Nlux

   Plasmid

   #176240

   Purpose

   pNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoter

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized lbCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Promoter

   Ribosomal subunit

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Myh6-Cas9

    Plasmid

    #109037

    Purpose

    Cardiac-specific overexpression of Cas9

    Depositor

    Eva van Rooij

    Article

    Johansen et al Circ Res. 2017 Oct 27;121(10):1168-1181.

    Insert

    Cas9
    

    Tags

    3XFlag

    Expression

    Mammalian

    Promoter

    alpha MHC

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCas9-T2A-mApple

    Plasmid

    #193780

    Purpose

    Tricistronic expression of Cas9-mApple-BSD in mammalian cells

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    mApple
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    In frame with Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AA008

    Plasmid

    #216003

    Purpose

    Fragmid fragment: (Cas protein) preferred over wildtype Cas12a

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas12a_v1.1 [EnAs]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-Blasticidin

    Plasmid

    #187954

    Purpose

    FKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-SpdCas9-hHDAC4-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC009 LshCas13a locus with targeting spacer

    Plasmid

    #91900

    Purpose

    LshCas13a locus into pACYC184 with targeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LshCas13a locus with targeting spacer
    

    Expression

    Bacterial

    Available Since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

    Plasmid

    #135012

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Tags

    3x Flag, 3xFLAG (N terminal on insert), NLS, and …

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-N-Blast-3xHA-LMNB1

    Plasmid

    #207778

    Purpose

    Donor template for Blast-2A-3xHA insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Blast-3xHA Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. qTAG-N-Puro-sTagRFP-TUBA1B

    Plasmid

    #207769

    Purpose

    Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. Cas12f-GE ver4.0

    Plasmid

    #176543

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver4.0
    Cas12f-GE ver4.0
    

    Expression

    Mammalian

    Promoter

    U6 and chicken β-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

    Plasmid

    #140198

    Purpose

    Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    dLbCas12a(D156R)-VPR
    

    Use

    CRISPR and Synthetic Biology; Expression in asper…

    Tags

    VPR (VP64-p65-Rta), NLS

    Mutation

    D832A DNase deactivated, D156R for improved activ…

    Promoter

    PgpdA

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-mitf

    Plasmid

    #69801

    Purpose

    Used to make mitf knockout porcine cell line

    Depositor

    Jianguo Zhao

    Article

    Wang et al Sci Rep. 2015 Aug 21;5:13348. doi: 10.10

    Inserts

    mitf-sgRNA
    SpCas9
    

    Expression

    Mammalian

    Promoter

    Chicken Beta-Actin and U6

    Available Since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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