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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 128,640 results for: CIL

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Showing: 10821 - 10840 of 128640 results

  5. NOTCH3-bio-His

    Plasmid
    #53343
    Purpose
    Expresses full-length Neurogenic locus notch homolog protein 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    NOTCH3 (NOTCH3 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53343-plasmid-map-sequence-id-83073

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  12. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191796
    Purpose
    vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized dCas13X
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    R84A, H89A, R739A, R740A, H745A, H746A, H747A
    Promoter
    CBh, SV40, hU6
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191796-plasmid-map-sequence-id-375578

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  14. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid
    #140980
    Purpose
    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphasS-RLuc8
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140980-plasmid-map-sequence-id-300680

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  17. pcDNA 3.1 SIRT1-FL

    Plasmid
    #105670
    Purpose
    Mammalian expression construct of mouse SIRT1 full length isoform
    Depositor
    Ullas Kolthur-Seetharam
    Publication
    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SIRT1 (Sirt1 Mouse)
    Expression
    Mammalian
    Mutation
    Synonymous base changes not affecting the protein…
    Promoter
    CMV
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105670-plasmid-map-sequence-id-207902

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  18. Fireworks GFP[PTC-] (AVA2598)

    Plasmid
    #85445
    Purpose
    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.
    Depositor
    Joan Steitz
    Publication
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Expression
    Mammalian
    Available since
    Feb. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    85445-plasmid-map-sequence-id-171491

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Showing: 10821 - 10840 of 128640 results