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pKI-cjOCT4-Cyan-pac

Plasmid

#160871

Purpose

Marmoset Targeting

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Res. 2021 Mar 24;53:102308. do

Insert

mCerulean3

Use

Marmoset targeting

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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OfCasp3b

Plasmid

#166054

Purpose

a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6

Depositor

Clay Clark

Article

Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

Insert

Orbicella faveolata caspase-3b

Tags

His tag

Expression

Bacterial

Promoter

T7 lac promoter

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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OfCasp3a

Plasmid

#166053

Purpose

a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspase

Depositor

Clay Clark

Article

Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

Insert

Orbicella faveolata caspase-3a

Tags

His tag

Expression

Bacterial

Promoter

T7 lac promoter

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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VTEHDTLLY_epitope

Plasmid

#165017

Purpose

Expression of CMV UL44 (245-253) fused to IL-2 signal sequence

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL44 (245-253)

Use

Lentiviral

Tags

human IL2 signal sequence at N-term

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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NTTVTFLPTPSLILQ_epitope

Plasmid

#165020

Purpose

Expression of CMV UL44 (37-51) fused to CD74

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL44 (37-51)

Use

Lentiviral

Tags

CD74 at N-term; cathepsin S cleavage site (GRWHTV…

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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SPIC_pet28a

Plasmid

#131654

Purpose

Bacterial expression of ETS gene SPIC

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPIC (SPIC Human)

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfKitlgb

Plasmid

#140293

Purpose

Express soluble His-tagged zebrafish Kitlgb in insect cells

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlgb (kitlgb Zebrafish)

Tags

6x His

Expression

Insect

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-sTomato. PGK-puro

Plasmid

#136264

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

sTomato

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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as-mOPN-PB0

Plasmid

#106450

Purpose

Bacterial expression of antisense to murine osteopontin

Depositor

Georg Weber

Article

Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

Insert

antisense-osteopontin (Spp1 Mouse)

Use

Retroviral

Mutation

antisense

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-AM

Plasmid

#153427

Purpose

Retroviral (MSCV) expression of ametrine fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

ametrine

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-LO

Plasmid

#153428

Purpose

Retroviral (MSCV) expression of LSSmOrange fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

LSSmOrange

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-HxHLICNot

Plasmid

#140183

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 12xHis-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

12xHis

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP

Plasmid

#124181

Purpose

piggyBac-based reporter (Puro) driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

IRES-Pac, Pou5f1 enhancer

Use

Reporter

Promoter

UBCmin

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP

Plasmid

#124180

Purpose

piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

EGFP-IRES-Pac, Pou5f1 enhancer

Use

Reporter

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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LOC5570372 NPFF receptor

Plasmid

#115378

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5570372 NPFF receptor (LOC5570372 Aedes aegypti)

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pWPI-TFDP2

Plasmid

#114300

Purpose

Constitutive expression of the TFDP2 protein

Depositor

Patrick Salmon

Article

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

TFDP2 (TFDP2 Human)

Use

Lentiviral

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pQE80L TriCatcher-MMP

Plasmid

#112632

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-MMP

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

central matrix metalloproteinase-cleavable sequen…

Promoter

T5

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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LOC5574680 Atrial natriuretic peptide receptor

Plasmid

#115374

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5574680 Atrial natriuretic peptide receptor (LOC5574680 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Rab1ab cds in pGem-T Easy

Plasmid

#86218

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

Rab1ab (rab1ab Zebrafish)

Use

In situ probe/other

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTD125 GFP:POP1

Plasmid

#71387

Purpose

localization of yeast RNase POP1 (RNase MRP binding protein) in yeast

Depositor

Mark Schmitt

Article

Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

Insert

POP1 (POP1 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

ACT1

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only