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AF10_OM-LZ

Plasmid

#111753

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

MLLT10 (MLLT10 Human)

Tags

6x His - TEV

Expression

Bacterial

Mutation

Contains the OM-LZ region of AF10 (residues 720-7…

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC3

Plasmid

#111754

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

Contains coiled coil #3 (residues 613-661)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC1

Plasmid

#111756

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

contains coiled coil #1 (residues 506-555)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DOT1L-CC2

Plasmid

#111755

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Article

Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

Insert

DOT1L (DOT1L Human)

Tags

8xHis-Thrombin-SUMO-TEV

Expression

Bacterial

Mutation

Contains coiled coil #2 (residues 560-608)

Promoter

T7

Available Since

June 14, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLuc-Optop38i-dsm

Plasmid

#89748

Purpose

Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i dark-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

C450A mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLuc-Optop38i-lsm

Plasmid

#89747

Purpose

Expression of light-regulated p38 inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i lit-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

I539E mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLuc-OptoJNKi-lsm

Plasmid

#89746

Purpose

Expression of light-regulated JNK inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi lit-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

I539E mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLuc-OptoJNKi-dsm

Plasmid

#89745

Purpose

Expression of light-regulated JNK inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi dark-state mutant

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

C450A mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFP

Plasmid

#91884

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFP

Plasmid

#91877

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F1)-GFP

Plasmid

#91883

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pMV-TRP1

Plasmid

#65335

Purpose

standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TRP1

Use

Synthetic Biology

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestGFP_RBPMS2

Plasmid

#65747

Purpose

expresses GFP-tagged RBPMS2 in mammalian cells

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS2 (RBPMS2 Human)

Tags

GFP

Expression

Mammalian

Mutation

S13N mutation in RBPMS2

Promoter

CMV, 2x Tet

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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mCardinal-CAF1-C-10

Plasmid

#56153

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

CAF1

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
CMV-sgE1TSS-short-3'box

Plasmid

#92165

Purpose

sgRNA expression vector for Evx1as RNA tethering assay

Depositor

Xiaohua Shen

Article

Luo et al Cell Stem Cell. 2016 May 5;18(5):637-52.

Insert

Evx1as short isoform

Use

CRISPR

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pET-Duet-ECHA(37-763) K351R-FLAG (no His-tag)

Plasmid

#96888

Purpose

expresses-flag tagged mouse ECHA(37-768) K351R in E.coli cells (no His-tag)

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)

Tags

FLAG-tagged

Expression

Bacterial

Mutation

deleted amino acid 1-36, mutated lysine 351 to ar…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pBabe-EGFP-Hu_Plk1(WT)

Plasmid

#136323

Purpose

Retroviral expression of human Plk1-WT with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Insert

PLK1 (PLK1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG-SLC38A9.1 (1-119aa)

Plasmid

#71862

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, first 119 amino acids

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pGL3-mFgf21pro3-luc

Plasmid

#101799

Purpose

Reporter for mouse Fgf21 promoter activity

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

Fibroblast growth factor 21 promoter (Fgf21 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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hsDCX-EGFP-pHAT

Plasmid

#83918

Purpose

human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II

Depositor

Gary Brouhard

Article

Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

Insert

Doublecortin isoform b (DCX Human, NM_178152.2)

Tags

6-His and EGFP+Strep-tag II

Expression

Bacterial

Available Since

Dec. 8, 2016

Availability

Academic Institutions and Nonprofits only

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