Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 25,303 results for: crispr

Clear keywords
Showing: 10821 - 10840 of 25303 results

  1. pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC

    Plasmid
    #140203
    Purpose
    Fungal vector to express an LbCas12a crRNA array targeted to Pelca, to be contransformed with pCRI009 containing PelcA-mCherry in a dLbCas12a-VPR strain as proof-of-concept of CRISPRa.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    crRNA array elcA
    Use
    CRISPR and Synthetic Biology
    Promoter
    PgpdA
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    140203-plasmid-map-sequence-id-310119

    Enlarge

  2. sg1+sg2+sg3

    Plasmid
    #113969
    Purpose
    Triple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC
    Depositor
    Jordan Green
    Publication
    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sg1+sg2+sg3
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    113969-plasmid-map-sequence-id-222998

    Enlarge

  6. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

    Enlarge

  8. pHybr_r2a>mG2a-srt-his

    Plasmid
    #124803
    Purpose
    HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Publication
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Murine IgG2a
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124803-plasmid-map-sequence-id-244318

    Enlarge

  10. pMT423

    Plasmid
    #139382
    Purpose
    Constitutively expressed dCas9, LacI and TetR, pNBU2 backbone, AmpR, TetR
    Depositor
    Christopher Voigt
    Publication
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Constitutively expressed dCas9, LacI and TetR
    Use
    Synthetic Biology
    Promoter
    P1
    Available since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    139382-plasmid-map-sequence-id-313187

    Enlarge

  13. FnoCas12a-2C-NLS in pCSDest

    Plasmid
    #126639
    Purpose
    Expresses FnoCas12a-2C-NLS in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FnoCas12a-2C-NLS
    Use
    CRISPR
    Tags
    3xHuman influenza hemagglutinin (HA)-Tag
    Expression
    Mammalian
    Promoter
    CMV IE94 promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    126639-plasmid-map-sequence-id-247958

    Enlarge

Showing: 10821 - 10840 of 25303 results