We narrowed to 11,662 results for: kars;

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pAAV-EF1a-fDIO-Cre

Plasmid

#121675

Purpose

Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

Depositor

Esteban Engel, Alexander Nectow

Article

Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

Has Service

AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

Insert

NLS-CRE-HA

Use

AAV

Tags

3xHA and SV40-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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1GFP/RNase H1 WT

Plasmid

#174447

Purpose

Express GFP-tagged RNase H1 WT in E. coli.

Depositor

Karlene Cimprich

Article

Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

RNase H1 (RNASEH1 Human)

Tags

His-tag, GFP

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-mCherry-IRES-Cre

Plasmid

#55632

Purpose

Expresses Cre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV8

Inserts

mCherry
Cre

Use

AAV

Expression

Mammalian

Promoter

Ef1a and Ef1a/IRES

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

Plasmid

#55639

Purpose

Flp-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde

Insert

hChR2(H134R)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-EGFPL10a

Plasmid

#98747

Purpose

Cre-dependently expresses the fusion protein EGFPL10a

Depositor

Nathaniel Heintz, Alexander Nectow, Eric Schmidt

Article

Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

Has Service

AAV Retrograde and AAV5

Insert

EGFPL10a

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry

Plasmid

#35504

Purpose

AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

C128S and D156A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon TVA-mCherry

Plasmid

#131779

Purpose

INTRSECT 2.0

Depositor

Karl Deisseroth

Article

Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

Insert

TVA-mCherry

Use

AAV; Intrsect

Tags

mCherry

Expression

Mammalian

Promoter

nEF

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-hMIGA2-DsRed

Plasmid

#192866

Purpose

Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

DsRed

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -0

Plasmid

#173886

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131004

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -2

Plasmid

#173888

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Fibronectin-human-plasma in pMAX

Plasmid

#120401

Purpose

enables eukaryotic expression of human plasma fibronectin

Depositor

Inaam Nakchbandi

Article

Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

Insert

Fibronectin (FN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -1

Plasmid

#173887

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-hTRF2

Plasmid

#103804

Purpose

BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hTRF2 (CIB1, TERF2 Mustard Weed, Human)

Expression

Mammalian

Mutation

hTRF2: deletion of amino acids 1-42 and 476 compa…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC476 - pAAV c-fos eYFP

Plasmid

#47907

Purpose

An AAV vector that expresses eYFP under the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

enhanced Yellow Fluorescent Protein

Use

AAV

Promoter

c-fos

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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AcGFP-MSH3

Plasmid

#215122

Purpose

Expresses AcGFP-MSH3 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

MSH3 (MSH3 Human)

Use

Retroviral

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pdCas9-GFP

Plasmid

#181906

Purpose

Fluorescently tagged dead Cas9

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

dCas9

Use

CRISPR

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-hPMLIII

Plasmid

#181903

Purpose

Fluorescently tagged PMLIII

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

hPMLIII (PML Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; HE1A:BFP -2

Plasmid

#180009

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; HE1A: BFP

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-MUTHY

Plasmid

#215132

Purpose

Expresses mCherry-MUTHY in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

MUTHY (MUTYH Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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