We narrowed to 13,472 results for: sequence
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Plasmid#147890PurposeMammalian Expression of HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAresDepositorInsertHsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres (CNOT1 Human)
ExpressionMammalianMutation1 silent mutation compared to the sequence of iso…Available SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1318-1605-IKGIRQtoDSYDAA_W
Plasmid#147894PurposeMammalian Expression of HsNot1iso1_1318-1605-IKGIRQtoDSYDAADepositorInsertHsNot1iso1_1318-1605-IKGIRQtoDSYDAA (CNOT1 Human)
ExpressionMammalianMutation1 silent mutation compared to the sequence of iso…Available SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Neo
Plasmid#186672PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Neomycin (YTHDF2 Human)
ExpressionMammalianAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Hygro
Plasmid#186668PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Hygromycin (ANKRD1 Human)
ExpressionMammalianAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cas9_ANKRD1_sgRNA2
Plasmid#186669PurposePlasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.DepositorInsertANKRD1 KO sgRNA Plasmid (ANKRD1 Human)
ExpressionMammalianAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4 TAPBPR-TM-TN6
Plasmid#178650PurposeMammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, R207E, Q209S, Q272S)DepositorInsertTAPBPR (TAPBPL Human)
TagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationE205K, R207E, Q209S, Q272S, switched transmembran…PromoterCMVAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-hFAM3B-HA
Plasmid#180145PurposeCoding sequence of human FAM3B pCS2 with C terminal HA tagDepositorAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-HsSyx3
Plasmid#179289PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage siteDepositorInsertSyx
TagsHis6 + GFP + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHsSyx3-MBP
Plasmid#179286PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6DepositorInsertSyx
TagsTEV protease cleavage site + mature maltose bindi…ExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-hFAM3B-FLAG
Plasmid#180146PurposeCoding sequence of human FAM3B in pCS2 with C terminal FLAG tagDepositorAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-RNFtoAAA_X
Plasmid#148000PurposeMammalian Expression of HsNot1iso1_840-1605-del1097-1110-RNFtoAAADepositorInsertHsNot1iso1_840-1605-del1097-1110-RNFtoAAA (CNOT1 Human)
ExpressionMammalianMutation1 silent mutation compared to the sequence of iso…Available SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-V75DL79D-GB1_AB
Plasmid#148379PurposeBacterial Expression of DmThor_50-83-V75DL79DDepositorInsertDmThor_50-83-V75DL79D (Thor Fly)
ExpressionBacterialMutationtwo silent mutations compared to the sequence giv…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dfnCas12a-KRAB_crRNA Td2
Plasmid#176261PurposepNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsKruppel associated box domain and luciferaseMutationD917A and E1006A mutations to inactivate the endo…Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 4
Plasmid#175815PurposepENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 4 (AT1G09270) without stop codon for C-terminal epitope tagsDepositorInsertIMPORTIN ALPHA ISOFORM 4 (IMPA-4 Mustard Weed)
UsePentr plasmid for gateway cloningTagsnonePromoternoneAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-503-WPRE
Plasmid#174133PurposepAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-503 fusion protein under the hSyn promoterDepositorInsertNos1ap (Nos1ap Mouse)
UseAAVTags3xFLAG and mCherryMutationOnly contains the sequences coding for amino acid…PromoterhSynAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-427-WPRE
Plasmid#174135PurposepAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-427 fusion protein under the hSyn promoterDepositorInsertNos1ap (Nos1ap Mouse)
UseAAVTags3xFLAG and mCherryMutationOnly contains the sequences coding for amino acid…PromoterhSynAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
PMP34-hLOV-TEVcs-GAL4bd-VP64-V5
Plasmid#170997PurposeHiLITR transcription factor with full-length PMP34 targeting sequence (Peroxisome)DepositorInsertPMP34(FL)-NNES-hLOV-TEVcs(ENLYFQ-M)-GAL4bd-VP64-V5 (SLC25A17 Synthetic)
UseLentiviral and Synthetic BiologyTagsV5ExpressionMammalianPromoterEf1-alphaAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL
Plasmid#172200PurposeAAV sequence-specific control vector for TALE-Tet1S-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.DepositorInsertHA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL
UseAAV and Mouse Targeting; TaleTagsHA, NLS, and T2A-EGFPExpressionMammalianMutationtranscription regulatory domain removedPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-dt/CBA-EGFP-R106bT
Plasmid#163921PurposeServes as control to pLV-PGK-dt/CBA-EGFP-106bT.DepositorInsertdtomato and EGFP
UseLentiviralExpressionMammalianMutationAdded 4-tandem reverse orientation-mir-106b-targe…Available SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_1-590 POLI
Plasmid#131233PurposeMammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a 24 bp insertion within the Pol iota co…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only