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C1-FT-mCitrine-EAAARx8-CaaX

Plasmid

#155224

Purpose

MPAct with increased search radius

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pTFE3-donor

Plasmid

#112377

Purpose

CRISPR donor plasmid to tag human transcription factor TFE3 with GFP

Depositor

Kevin White

Insert

TFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)

Use

CRISPR

Mutation

hg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-PKI nls

Plasmid

#118301

Purpose

Expression of PKIa

Depositor

Philip Stork

Article

Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

Insert

PKI nls (aa 2-36)

Tags

Flag and GFP

Expression

Mammalian

Mutation

inserting 2xnls

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pH4R-P2A-mCherry-N1

Plasmid

#84333

Purpose

Co-expresses (untagged) Histamine-4 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H4R-P2A-mCherry (HRH4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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5HT2C-RNA1-FLAG

Plasmid

#79678

Purpose

serotonin receptor 2C RNA isoform 1 with C-terminal flag tag

Depositor

Stefan Stamm

Article

Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

Insert

5HT2C-RNA1 (HTR2C Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only
FH95pUp95GW (B4)

Plasmid

#74013

Purpose

lentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNA

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pNMHCII-C1C2-GFP

Plasmid

#46044

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

Insert

nonmuscle myosin heavy chain II-C, isoform C1C2 (Myh14 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV immediate early

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only
miniCGBE1-VRQR (pBM1173)

Plasmid

#140255

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)_VRQR-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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SECURE BE4max(R33A) (pBM608)

Plasmid

#140251

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSc2

Plasmid

#80437

Purpose

Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

Depositor

Ervin Welker

Article

Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

Inserts

sp Cas9 gRNA
eGFP

Use

CRISPR

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-132-Decoy.hPGK.GFP.WPRE

Plasmid

#46587

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-132 Decoy (MIR132, Mir132 Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pMXs-ERas CVLS-IP

Plasmid

#13828

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas CVLS (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

CSVA (224-227) changed to CVLS (CAAX sequence of …

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pUltra-MDH1-ME1

Plasmid

#184465

Purpose

Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Inserts

Malate dehydrogenase 1 (MDH1 Human)
Malic Enzyme 1 (ME1 Human)

Use

Lentiviral

Tags

fused to T2A

Expression

Mammalian

Mutation

deletion of stop codon

Promoter

UbC

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-FLT1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156962

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

FLT1 (FLT1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-PRMT9 L182A/D183A/I184A/G185A

Plasmid

#79676

Purpose

mammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L182A/D183A/I184A/G185A catalytic mutant

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-10a-Decoy.hPGK.GFP.WPRE

Plasmid

#46581

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-10a Decoy (MIR10A, Mir10a Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
LEGO-AP1x6-GM55

Plasmid

#217415

Purpose

Luciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x6

Depositor

Peter Cockerill

Insert

Luciferase, GFP

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVd1-NLS-GAL4-iLight2-VP16

Plasmid

#219550

Purpose

NLS-Gal4(1-65)-iLight2-VP16 construc expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16

Use

AAV

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac SpyTag-β2AR-SpyTag

Plasmid

#135620

Purpose

Expression of SpyTag-B2AR in insect cell

Depositor

Hee-Jung Choi

Article

Choi et al Science. 2019 Nov 29;366(6469):1150-1156

Insert

Beta 2 adrenergic receptor (ADRB2 Human)

Tags

HA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tag

Expression

Insect

Mutation

Deleted 1-24 aa, 366-413 aa

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-Aurora_CA

Plasmid

#98170

Purpose

slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct Aurora_C128A gene

Tags

mCherry

Expression

Mammalian

Mutation

V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

Promoter

CMV (+enhancer)

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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