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We narrowed to 14,949 results for: EGF

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Showing: 10901 - 10920 of 14949 results

  8. pB80-SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)

    Plasmid
    #174643
    Purpose
    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)
    Tags
    SSPB(nano)-GFP
    Expression
    Mammalian
    Mutation
    EGFP: Met1Del; ppKin14VIb(aa861-1321)
    Promoter
    Chicken beta-actin
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174643-plasmid-map-sequence-id-346994

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  13. Anti-LRP4 (extracellular) [N207/27R] (Recombinant Antibody)

    Antibody
    #184201-rAb
    Purpose
    Anti-LRP4 (extracellular) (Mouse) recombinant mouse monoclonal antibody
    Depositor
    James Trimmer
    Article
    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1
    Recommended Applications
    Immunocytochemistry, Immunohistochemistry, and Western Blot
    Reactivity
    Mouse and Rat
    Source Species
    Mouse
    Isotype
    IgG2a
    Trial Size
    Available to purchase
    Available Since
    Aug. 11, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by James Trimmer, UC Davis

  15. pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro)

    Plasmid
    #174630
    Purpose
    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVD52C-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174630-plasmid-map-sequence-id-346973

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  16. pCOTS-pyl-GFP(35TAG)

    Plasmid
    #92047
    Purpose
    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.
    Depositor
    Lital Alfonta
    Article
    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/
    Inserts
    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    Use
    Replicative expression plasmid for cyanobacteria …
    Tags
    Histag
    Mutation
    Tyrosine in position 35 of the GFP was mutated f…
    Promoter
    LeuP (native S. elongatus promoter), PpsbII, and …
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92047-plasmid-map-sequence-id-194905

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  17. FNL Combinatorial Cloning Platform

    Plasmid Kit
    #1000000211
    Purpose
    A Frederick National Laboratory (FNL) combinatorial cloning set to generate many tagged expression clones in parallel for protein production, in vivo gene expression, and shRNA and CRISPR delivery.
    Depositor
    Dominic Esposito
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Bacterial Expression, Insect Expression, Mammalian Expression
    Cloning Type
    MultiSite Gateway
    Available Since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only

  18. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)

    Plasmid
    #208957
    Purpose
    A variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208957-plasmid-map-sequence-id-415746

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  19. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29 (JO582)

    Plasmid
    #208954
    Purpose
    A variant CE1 construct with Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Phi29(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208954-plasmid-map-sequence-id-415743

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  20. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129028
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129028-plasmid-map-sequence-id-256131

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Showing: 10901 - 10920 of 14949 results