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pCas9-HE-Geminin

Plasmid

#109402

Purpose

Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron

Depositor

Jean-Paul Concordet

Article

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

HE-Geminin

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-yellow pre-eGRASP(p30)

Plasmid

#111588

Purpose

An AAV vector that expresses yellow pre-eGRASP(p30) under the tetO promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

yellow pre-eGRASP(p30)

Use

AAV

Promoter

tetO

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE3G-BE3RA-PGK-Puro

Plasmid

#110846

Purpose

Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Mutation

D10A

Promoter

TRE3G

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-mTurquoise2

Plasmid

#108235

Purpose

Broad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

Pc promoter - mTurquoise2

Expression

Bacterial

Mutation

codon optimized for experession in P. fluorescens

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-hcGAS(157-522)

Plasmid

#108676

Purpose

For expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

cGAS amino acid 157-522 (CGAS Human)

Tags

GST

Expression

Bacterial

Mutation

contains amino acid 157-522

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1

Plasmid

#108127

Purpose

Recruitable SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L (SACM1L Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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IFNAR1_pCSdest

Plasmid

#53881

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

IFNAR1 (IFNAR1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pFRT-TO-DEST

Plasmid

#106348

Purpose

Destination vector for gateway cloning. Developed for Flp-In T-REx system.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_ELAVL1

Plasmid

#106104

Purpose

Inducible gene expression of FlagHA ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ELAVL1 (ELAVL1 Human)

Tags

Flag and HA

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1 _3xHA-Ago2

Plasmid

#73538

Purpose

Inducible lentiviral expression of Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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GST-AR-624-919

Plasmid

#104200

Purpose

Expresses human AR-624-929 ligand binding domain in pGEX-5X-1 as GST fusion protein

Depositor

Elizabeth Wilson

Article

He et al Mol Cell Biol. 2003 Mar;23(6):2135-50.

Insert

human androgen receptor aa 624-919 (AR Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

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ECFP-TevS-YPET

Plasmid

#100097

Purpose

FRET-based ECFP-YPET reporter that is cleaved by TEV

Depositor

Jim Wells

Article

Gray et al Cell. 2010 Aug 20;142(4):637-46. doi: 10

Insert

ECFP-TevS-YPET

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pC020 - LseCas13a from Listeria seeligeri

Plasmid

#91910

Purpose

Expresses LseCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LseCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pKan-PCUP1-9myc-AID*(N)

Plasmid

#99527

Purpose

N-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Promoter

CUP1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-IV-GFPL10

Plasmid

#98704

Purpose

Cre-dependently express GFPL10 fusion using Introvert Switch

Depositor

Jeffrey Friedman, Alexander Nectow

Article

Nectow et al Cell. 2017 Jul 27;170(3):429-442.e11. do

Insert

GFPL10

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

L10: 2609 C->T to remove EcoRI site

Promoter

EF1a

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HR donor

Plasmid

#97317

Purpose

HR donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HR donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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8024 GFP_Kras_G12C_2A_puro

Plasmid

#64373

Purpose

This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2A oncogene along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2A (Kras Mouse)

Use

Retroviral

Tags

GFP

Mutation

changed glycine 12 to cysteine

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDHa_mDDX5_FL_wt

Plasmid

#88869

Purpose

constitutive expression of Ha-DDX5 in mammalian cells

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (Ddx5 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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L40C-CRISPR.EFS.mNeon

Plasmid

#69146

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-mNeonGreen

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl

Plasmid

#90485

Purpose

Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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