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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,021 results for: T7

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Showing: 10961 - 10980 of 17021 results

  5. pSLS.492

    Plasmid
    #184957
    Purpose
    Test effect of a1/a2 length on editing rpoB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
    Expression
    Bacterial
    Mutation
    rpoB donor T1534C, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184957-plasmid-map-sequence-id-376681

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  7. eSrtA(2A-9) pET29b

    Plasmid
    #75145
    Purpose
    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity
    Depositor
    David Liu
    Article
    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11
    Insert
    reprogrammed sortase A variant
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S102C A104H E105D K138P K152I N160K K162H T164N K…
    Promoter
    T7
    Available Since
    July 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    75145-plasmid-map-sequence-id-466935

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  9. Affibody EGFR:1907.BirA*-pET301/NT-DEST

    Plasmid
    #174691
    Purpose
    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*
    Depositor
    Jean Gariepy
    Article
    Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2
    Insert
    Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
    Tags
    HIS tag
    Expression
    Bacterial
    Mutation
    R118G mutation in BirA
    Promoter
    T7 promoter
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174691-plasmid-map-sequence-id-347065

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  15. eSrtA(4S-9) pET29b

    Plasmid
    #75146
    Purpose
    reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity
    Depositor
    David Liu
    Article
    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11
    Insert
    reprogrammed sortase A variant
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    N98D S102C A104V A118T F122A K134R F144L I182V E1…
    Promoter
    T7
    Available Since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    75146-plasmid-map-sequence-id-142065

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  18. pLyGo-Ec-1

    Plasmid
    #163127
    Purpose
    pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Ubiquitin
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    163127-plasmid-map-sequence-id-331491

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Showing: 10961 - 10980 of 17021 results