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Plasmid#145836PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody S1-54 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046647
Plasmid#153940PurposeYeast expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p667-UBC-AAK1-V5_IDG-K
Plasmid#135257PurposeGateway destination clone of AAK1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertAAK1-V5 (AAK1 Human)
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianPromoterUbiquitinAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Miro1 S156E
Plasmid#127589PurposePhospho-mimetic of S156DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMiro1 (RHOT1 Human)
TagseGFPExpressionMammalianMutationS156EAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
myc-Miro1 (lacking TM)
Plasmid#127613PurposeHuman Miro1 with the transmembrane deletedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMiro1 (RHOT1 Human)
TagsmycExpressionMammalianMutationDeletion of transmembrane domainAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MigR1-Flag-TRIB2
Plasmid#131154PurposeRetroviral expression of flag-tagged mouse TRIB2DepositorAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-Sac-Abeta(MC1-40)
Plasmid#127152PurposeExpresses N-terminal cysteine Abeta(1-40)DepositorAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rap1b S179AS180A
Plasmid#118327PurposeExpression of bovine Rap1bDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRap1b S179AS180A (RAP1B Bovine)
TagsGFPExpressionMammalianMutationS179AS180AAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pediculus humanus corporis PINK1 (143 - 575)
Plasmid#110751PurposeBacterial expression of PhPINK1 (crystallography)DepositorInsertPhum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
TagsN-His6-SUMOExpressionBacterialPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
vsv-Sgo1 N61I-GFP
Plasmid#108497Purposeexpression of vsv-Sgo1 N61I-EGFP (no PP2A binding)DepositorInsertShugoshin 1 (SGO1 Human)
TagsEGFP and VSVExpressionMammalianMutationno PP2A binding N61IPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-a (colE1 origin)
Plasmid#74091PurposeSecondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationDepositorPublicationInsertGolden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-b (colE1 origin)
Plasmid#74092PurposeSecondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationDepositorPublicationInsertGolden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC-20xUAS-TALE3-VP64-P10
Plasmid#104607PurposeExpresses TALE3 under UAS controlDepositorInsertTALE3
UseTALENExpressionInsectPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHT-497
Plasmid#87756PurposeBacterial expression vector for producing 6x histidine tagged rat DYRK1A kinase domain (residues 1-497)DepositorInsertGene coding for rat DYRK1A kinase domain (residues 1-497)
Tags6X histidine tagExpressionBacterialPromoterT7 RNA polymerase promoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEV-VMA Model vector
Plasmid#86590PurposeThis can be used to make MBP-TEV-Cterm with SapI and PstI; and can also make N-term-VMA with NdeI and SapIDepositorInsertVMA intein
TagsHis6-MBPExpressionBacterialMutationOriginal SapI site from pMal was mutated outPromoterTacAvailable sinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
KC1E_HUMAN_D0
Plasmid#79724PurposeThis plasmid encodes the kinase domain of KC1E. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + PrgJ
Plasmid#84869PurposeBaculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
PrgJ
UseBaculovirusTags6xHisExpressionInsectPromoterPolyhedrinAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
rFIXX-bp chain A (IRESneo)
Plasmid#82450PurposeChain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.DepositorInsertrFIXX-bp chain A
TagsPolyhistidineExpressionBacterial and MammalianPromoterT7 RNA polymerase promoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB118
Plasmid#68572PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsModified EAR motif plant-specific repression doma…ExpressionPlantPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntn3-Fc-His
Plasmid#72105PurposeExpresses the entire Netrin 3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by