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pLV 4xSTAR-mTFP-NLS-blast

Plasmid

#136257

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mTFP-NLS

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-mScarletI. PGK-puro

Plasmid

#136266

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

mScarletI

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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kermit/pCS2+

Plasmid

#16309

Depositor

Peter Klein

Article

Tan et al Development. 2001 Oct . 128(19):3665-74.

Insert

Kermit (gipc1 Frog)

Use

Vertebrate expression

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only

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Calnexin-HT-mGold

Plasmid

#183987

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells with localization in the ER.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

Calnexin (CANX Human)

Tags

HaloTag, mGold

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM60 FLAG-RAB35 S22N

Plasmid

#107106

Purpose

Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

Depositor

Charles Sawyers

Article

Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

Insert

RAB35 (RAB35 Human)

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

Plasmid

#216156

Purpose

Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with C-terminal FLAG tag

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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SHIP2 WT pBABE-His-Xpress

Plasmid

#126584

Purpose

Retroviral infection of MEF cells (puromycin selection)

Depositor

Christophe Erneux

Article

Zhang et al Cell Signal. 2007 Oct;19(10):2194-200. d

Insert

SHIP2 (INPPL1 Human)

Use

Retroviral

Tags

His Xpress (N-terminal)

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Muc2D3

Plasmid

#155216

Purpose

Expresses human Muc2 residues 858-1259

Depositor

Deborah Fass

Article

Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

Insert

Muc2 D3 (MUC2 Human)

Tags

6xHistadine

Expression

Mammalian

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mPapaya1.0

Plasmid

#52304

Purpose

An engineered monomeric Zoanthus sp. yellow fluorescent protein called mpapaya

Depositor

Robert Campbell

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

fluorescent protein mpapaya

Expression

Mammalian

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116955

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Trex2

Plasmid

#79246

Purpose

Expression vector for Trex2, which is a non-processive 3' exonuclease.

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2009 Oct;5(10):e1000683. doi

Insert

Trex2 (Trex2 Mouse)

Expression

Mammalian

Promoter

pCAGGS

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pET15b His-p97

Plasmid

#169020

Purpose

Expresses His-p97 in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pUC6

Plasmid

#49793

Purpose

E. coli cloning vector

Depositor

Joachim Messing

Article

Vieira et al Gene. 1982 Oct;19(3):259-68.

Type

Empty backbone

Use

Cloning vector

Promoter

none

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only
mPapaya-Lifeact-7

Plasmid

#54540

Purpose

Localization: Actin, Excitation: 530, Emission: 541

Depositor

Robert Campbell, Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Lifeact-mPapaya

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
Flag-FOXE1

Plasmid

#153120

Purpose

Expression of Flag tagged human FOXE1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXE1 (FOXE1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXF1

Plasmid

#153122

Purpose

Expression of Flag tagged human FOXF1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXF1 (FOXF1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAPM-D4- miR30-L1221

Plasmid

#115846

Purpose

Control shRNA KD vector

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

control shRNA

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Reckleen_1_Cas9

Plasmid

#233456

Purpose

Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 2 RECKLEEN plasmid

Use

CRISPR

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEHIShqprt

Plasmid

#92166

Purpose

Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

Quinolinate Phosphoribosyltransferase (QPRT Human)

Tags

TEV protease -cleavable N-terminal 6His

Expression

Bacterial

Mutation

changed Asp 2 to Asn to create BspHI site

Promoter

T7

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Lifeact_Halo_T2A_EGFP

Plasmid

#135445

Purpose

Mamalian cell expression of HaloTag7 localized at F-actin and cytosolic EGFP

Depositor

Kai Johnsson

Article

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Insert

Lifact-HaloTag7 and EGFP

Tags

Lifact

Expression

Mammalian

Promoter

CMV/TetO

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pBS chick PEA3 [TJ#51]

Plasmid

#16279

Depositor

Thomas Jessell

Article

Lin et al Cell. 1998 Oct 30. 95(3):393-407.

Insert

PEA3 (ETV4 Chicken)

Expression

Bacterial

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
KHBD00202

Plasmid

#34353

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG33261 (Trl Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pcold-6xHis-HRV3Csite-DmLoqsPA

Plasmid

#41093

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Loquacious-PA (loqs Fly)

Tags

6xHis-HRV3Csite

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 HA-FAM208A

Plasmid

#115845

Purpose

Encodes codon optimized HA tagged FAM208A

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HA tagged FAM208A (C3orf63 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pABY-T4

Plasmid

#201767

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR221-EF1.Pro

Plasmid

#79488

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

EF1 Promoter (EEF1A1 Synthetic, Human)

Use

Gateway Cloning

Promoter

EF1 Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2-del409-432_T

Plasmid

#147625

Purpose

Mammalian Expression of DmNot2-del409-432

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del409-432 (Rga Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot2-del344-380_T

Plasmid

#147629

Purpose

Mammalian Expression of DmNot2-del344-380

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del344-380 (Rga Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot2-del380-404_T

Plasmid

#147630

Purpose

Mammalian Expression of DmNot2-del380-404

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del380-404 (Rga Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot2-del409-432_T

Plasmid

#147631

Purpose

Mammalian Expression of DmNot2-del409-432

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del409-432 (Rga Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLNHA-C1-HsNot2-del344-380_T

Plasmid

#147623

Purpose

Mammalian Expression of DmNot2-del344-380

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del344-380 (Rga Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLNHA-C1-HsNot2-del380-404_T

Plasmid

#147624

Purpose

Mammalian Expression of DmNot2-del380-404

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del380-404 (Rga Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPN170

Plasmid

#91599

Purpose

Express sgRNA targeting human DLGAP1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DLGAP1 (DLGAP1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPN171

Plasmid

#91600

Purpose

Express sgRNA targeting human DLGAP1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DLGAP1 (DLGAP1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
3 gRNA concatemer

Plasmid

#84880

Purpose

Empty concatmer vector in which 3 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only
EC71167

Plasmid

#154066

Purpose

Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct

Use

Synthetic Biology

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Promoter

Zea mays Ubiquitin Promoter

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM60 FLAG-RAB35 Q67L

Plasmid

#107105

Purpose

Lentiviral vector that codes for FLAG-RAB35 Q67L (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

Depositor

Charles Sawyers

Article

Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

Insert

RAB35 (RAB35 Human)

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
KHBD00703

Plasmid

#39739

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3905 (Su(z)2 Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00596

Plasmid

#39677

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6061 (mip120 Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00286

Plasmid

#34412

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG18647 (bin Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only

Showing: 2161 - 2200 of 4444 results

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