We narrowed to 32,433 results for: Eng;
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Plasmid#110593PurposeBTK Type 6-7 terminator (replaces Type 6 and Type 7 parts)DepositorInsertBba_B0015 Terminator
UseSynthetic BiologyAvailable SinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-MBP-CBX1-eReader
Plasmid#135089PurposeBacterial expression of engineered CBX1 with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered
Tags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK519
Plasmid#110603PurposeBTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance)DepositorInsertGFP
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-ndufs2 guide
Plasmid#107601PurposeAn entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expressionDepositorInsertndufs2 guide RNA sequences
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-INPP4A
Plasmid#171944PurposeExpresses INPP4A in mammalian cellsDepositorAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-40XUAS-Luc
Plasmid#166455PurposeLuciferase reporterDepositorInsert40XUAS , Luciferase
UseLentiviralAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
iCAP-BI155-KanR
Plasmid#209527PurposeRepCap for AAV productionDepositorInsertAAV-BI155 Cap
ExpressionMammalianAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
BPK2301
Plasmid#65778PurposeHuman expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNADepositorInsertSt1Cas9 gRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMBA333
Plasmid#214745PurposeMedium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3
ExpressionBacterialAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK402
Plasmid#110598PurposeBTK Type 8 Bacterial origin (broad-host-range) for golden gate assemblyDepositorInsertmRFP dropout (RSF1010 origin)
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-rac2 guide
Plasmid#107600PurposeAn entry vector with U6a and U6c promoter driving rac2 guide RNAs expressionDepositorInsertrac2 guide RNA sequences
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Fyn-Fyn Biosensor
Plasmid#124655PurposeFRET biosensor. Fyn-tagged biosensor targeting to membraneDepositorInsertSynthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
ExpressionMammalianAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK501
Plasmid#110602PurposeBTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)DepositorInsertGFP
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSP977
Plasmid#65774PurposeHuman expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135E mutation in Cas9PromoterCMV & T7Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBTK229
Plasmid#110591PurposeBTK Type 3 coding sequence for golden gate assembly and homologous recombinationDepositorInsertKanamycin resistance
UseSynthetic BiologyAvailable SinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK570
Plasmid#110615PurposeBTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoterDepositorInsertE2-Crimson
ExpressionBacterialAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK520
Plasmid#110604PurposeBTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance)DepositorInsertGFP
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK510
Plasmid#110611PurposeBTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoterDepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CMV-mTurquoise2-NLS
Plasmid#165443PurposeExpresses nuclear mTurquoise2 from CMV promoterDepositorInsertmTurquoise2-NLS
UseAAVTagsNLSExpressionMammalianPromoterCMVAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK564
Plasmid#110613PurposeBTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoterDepositorInsertNanoluc
ExpressionBacterialAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK599
Plasmid#110600PurposeBTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombinationDepositorInsertGFP dropout
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSUPERIORpuro-shGAS5
Plasmid#46370DepositorAvailable SinceAug. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262m3
Plasmid#149556PurposeGateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMsDepositorInsertecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
UseCRISPRExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domai…Available SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ensconsin-18-mEos2
Plasmid#99230PurposeMammalian expression of the photoconvertible protein mEos2 as an Ensconsin fusion.DepositorInsertmEos2
TagsEnsconsin (MAP7)ExpressionMammalianPromoterCMVAvailable SinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBTK509
Plasmid#110610PurposeBTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoterDepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
POT2-RFP
Plasmid#65318Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBTK121
Plasmid#110581PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertPA3 Promoter with RBS
UseSynthetic BiologyAvailable SinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMV-HIS3
Plasmid#65333Purposestandard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUsDepositorInsertHIS3
UseSynthetic BiologyAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
NG0886 pCI-TPI-WT-4H
Plasmid#65801PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
Tags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBTK615
Plasmid#110609PurposeBTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9DepositorInsertsgRNA
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTX209
Plasmid#89269PurposeExpress STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02DepositorInsertOsPDS-gRNA01 and OsPDS-gRNA02
UseCRISPRTagsSV40 NLSExpressionPlantPromoterMaize Ubiquitin1 promoterAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQCas12j2
Plasmid#189780PurposeGateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoterDepositorInsertCas12j2
UseCRISPR; Gateway compatible cas12j2 entry cloneTagsNLSExpressionPlantAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-RNF169
Plasmid#99392PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169DepositorInsertRNF169 (RNF169 Human)
TagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
POT4-RFP
Plasmid#65320Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBTK563
Plasmid#110612PurposeBTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoterDepositorInsertNanoluc
ExpressionBacterialAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK124
Plasmid#110583PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertCP25 promoter with lac operator and RBS
UseSynthetic BiologyAvailable SinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK403
Plasmid#110599PurposeBTK Type 8 Bacterial origin (broad-host-range) for golden gate assemblyDepositorInsertmRFP dropout (RSF1010 origin)
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK401
Plasmid#110597PurposeBTK Type 8 Bacterial origin (broad-host-range) for golden gate assemblyDepositorInsertmRFP dropout (RSF1010 origin)
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK130
Plasmid#110584PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertT7 promoter with lac operator and RBS
UseSynthetic BiologyAvailable SinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK550d
Plasmid#110617PurposeBTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymeraseDepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcold-6xHis-HRV3Csite-DmLoqsPA
Plasmid#41093DepositorAvailable SinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUHG10.3-TetO-Rs1
Plasmid#16313DepositorInserth5HT4b Serotonin Receptor (Rs1 D100A RASSL) (RS1 Human)
TagsFLAGExpressionMammalianMutationAdded FLAG tag; Changed Aspartic Acid 100 to Alan…Available SinceJan. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
POT10-RFP
Plasmid#65326Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT8-RFP
Plasmid#65324Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT7-RFP
Plasmid#65323Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT6-RFP
Plasmid#65322Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-eReader
Plasmid#135090PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered x3
Tags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-CU2-Pleum
Plasmid#60328PurposeContains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK599s
Plasmid#110601PurposeBTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombinationDepositorInsertGFP dropout
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
UASGAL-PCYC
Plasmid#60335PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits