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pcDNA3.1-Hyg-mEGFP-pp4640

Plasmid

#191846

Purpose

Expresses Salmon Alphavirus polyprotein with mEGFP N-terminal fusion

Depositor

Bertrand Collet

Article

Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

Insert

mEGFP-pp4640

Tags

mEGFP

Expression

Mammalian

Mutation

mEGFP fusion in Nterminal, some silent point muta…

Promoter

CMV

Available Since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

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bu6-sgCebpa_v1-mU6-sgCebpb_v1-hU6-sgCebpd_v1

Plasmid

#177257

Purpose

Expresses Cebpa_v1 (bU6), Cebpb_v1 (mU6), Cebpd_v1 (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_v1/sgCebpb_v1/sgCebpd_v1

Use

Lentiviral

Promoter

bU6/mU6/hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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bu6-sgCebpa_v2-mU6-sgCebpb_v2-hU6-sgCebpd_v2

Plasmid

#177258

Purpose

Expresses Cebpa_v2 (bU6), Cebpb_v2 (mU6), Cebpd_v2 (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_v2/sgCebpb_v2/sgCebpd_v2

Use

Lentiviral

Promoter

bU6/mU6/hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

Plasmid

#62577

Purpose

Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pMIR-Report-RASA1-3'UTR

Plasmid

#62575

Purpose

Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

RASA1 (RASA1 Human)

Use

Luciferase

Tags

None

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pUT1141-HQ858685

Plasmid

#27731

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

bZIP transcription factor from EE179215

Use

Gateway Cloning

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1439-HQ858741

Plasmid

#27823

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

NAC transcription factor from DT947867

Use

Gateway Cloning

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1455-HQ858749

Plasmid

#27831

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

ORPHAN transcription factor from DR801947

Use

Gateway Cloning

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1682-HQ858766

Plasmid

#27848

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

GRAS transcription factor from DV163953

Use

Gateway Cloning

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only
pUT1769-HQ858783

Plasmid

#27865

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

DBP transcription factor from EB701836

Use

Gateway Cloning

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pspCas9-Klenow-ST2-com-vector

Plasmid

#176235

Purpose

Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Type

Empty backbone

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-gcry-eGFP

Plasmid

#117791

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-TagBFP

Plasmid

#117765

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-gcry-mRFP

Plasmid

#117772

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB3035(X-4 MarkerFree)

Plasmid

#73273

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2909(XII-5 MarkerFree)

Plasmid

#73281

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PGL4.11-COL1A1mTEAD

Plasmid

#230916

Purpose

Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.

Depositor

Avi Rosenberg

Article

Yang et al Lab Invest. 2024 May;104(5):100336. doi:

Insert

Human COL1A1 promoter with mutant TEAD elements (COL1A1 Human)

Use

Luciferase

Tags

Luciferase- luc2p

Expression

Bacterial and Mammalian

Mutation

mutant potential TEAD bind elements AGGAAT to CTG…

Promoter

COL1A1

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLVNP3.0-ABE8e

Plasmid

#206882

Purpose

Expresses FLAG-tagged ABE8e fused to Gag through a linker sequence

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

ABE8e

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-mRFP

Plasmid

#117793

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-gcry-mRFP

Plasmid

#117778

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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