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  11. KTD101(DE3)

    Bacterial Strain
    #138651
    Purpose
    KTD101(DE3) is a trigger factor deficient strain, which may increase protein secretion from Escherichia coli, and is compatible with expression from the T7 promoter
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Bacterial Resistance
    None
    Species
    Escherichia coli
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only

  15. pQE80L-SpyCatcher-ELP-GFP

    Plasmid
    #69835
    Purpose
    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyCatcher-ELP-GFP
    Use
    Tags
    6x His Tag, GFP, and TEV Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter/lac operator element
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69835-plasmid-map-sequence-id-183106

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  19. pLenti-CaMKIIa-SpyTag-C1C2-mCherry

    Plasmid
    #69833
    Purpose
    Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing SpyTag-C1C2-mCherry fusion. SpyTag can be used to monitor membrane localization of the C1C2 opsin.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyTag-C1C2
    Use
    Lentiviral
    Tags
    Trafficking signal and mCherry
    Expression
    Mammalian
    Mutation
    Inserted SpyTag after the signal peptide of C1C2.
    Promoter
    CamKIIa
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69833-plasmid-map-sequence-id-183098

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  20. CD8a-hnRNP-M-EGFP

    Plasmid
    #86054
    Purpose
    A mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    CD8a (CD8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    CD8a luminal and transmembrane domians along with…
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86054-plasmid-map-sequence-id-177920

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Showing: 1081 - 1100 of 16731 results