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YIp LYS2-HIS3

Plasmid

#122908

Purpose

LYS2-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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iRFP713-C1-SNAP-Fis1tail

Plasmid

#220067

Purpose

allows for a reversible dimerization system to be targeted at the mitochondria with an iRFP fluorophore

Depositor

Gerry Hammond

Publication

Doyle et al Contact (Thousand Oaks). 2024 Feb 22;7:2

Insert

SNAP-Fis1tail

Expression

Mammalian

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET-mNeonGreen-HMG-15b

Plasmid

#206872

Purpose

Express mNeonGreen-HMG

Depositor

Kyubong Jo

Publication

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

mNeonGreen-HMG

Expression

Bacterial

Promoter

T7 promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-MET15

Plasmid

#207013

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae)

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Im7 WT

Plasmid

#201081

Purpose

Im7 WT inserted into CysGA at G364 via GS linker

Depositor

Shu Quan

Publication

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Im7

Expression

Bacterial

Promoter

pTrc

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-TP-UnaG

Plasmid

#203484

Purpose

For particle bombardment. A plastid transit peptide fused UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Publication

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG

Expression

Bacterial and Plant

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_69.11

Plasmid

#194214

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Publication

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Expression

Bacterial

Available since

July 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Publication

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Expression

Mammalian

Available since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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mouse ZNG1

Plasmid

#197399

Purpose

expresses tagless mouse ZNG1 in the pHIS vector (inserted at NdeI-BamHI)

Depositor

David Giedroc

Publication

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

ZNG1 (Cbwd1 Mouse)

Expression

Bacterial

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-dCer3

Plasmid

#191328

Purpose

Expresses the fluorescently labeled membrane anchor C2 without the chemical dimerization domain FRB as a control for recruiting proteins to plasma membrane

Depositor

Frank Bradke

Publication

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

dCer3-C2-dCer3

Expression

Mammalian

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pE5772

Plasmid

#170779

Purpose

expression of hybrid proteins in E. coli

Depositor

Anthony Schryvers

Publication

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

GentR

Tags

polyhisbioMbpTEV

Expression

Bacterial

Promoter

T7

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pZS181

Plasmid

#177637

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Publication

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

HaloTag

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pZS212

Plasmid

#177639

Purpose

In vitro transcription of Patiria miniata Lamp1

Depositor

Iain Cheeseman

Publication

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Lamp1

Use

In vitro transcription

Tags

mCherry

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pZS217

Plasmid

#177640

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Publication

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pZS222

Plasmid

#177644

Purpose

In vitro transcription of Patiria miniata Lamp1

Depositor

Iain Cheeseman

Publication

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Lamp1

Use

In vitro transcription

Tags

3xGFP

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pZS218

Plasmid

#177641

Purpose

In vitro transcription of Patiria miniata Rab7

Depositor

Iain Cheeseman

Publication

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Rab7

Use

In vitro transcription

Tags

mCherry

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pZS230

Plasmid

#177645

Purpose

Bacterial expression of Patiria miniata Dishevelled 1-90

Depositor

Iain Cheeseman

Publication

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled 1-90

Tags

GST

Expression

Bacterial

Available since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pZS202

Plasmid

#177638

Purpose

In vitro transcription of Patiria miniata Frizzled 1

Depositor

Iain Cheeseman

Publication

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Frizzled 1

Use

In vitro transcription

Tags

GFP

Available since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-LifeAct-EGFP

Plasmid

#163608

Purpose

Expresses LifeAct fused to EGFP Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

LifeAct

Tags

EGFP

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Cdc42

Plasmid

#163606

Purpose

Expresses Cdc42 Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Cdc42 (Cdc42 Mouse)

Expression

Mammalian

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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