168,234 results

1. pLL3.7

   Plasmid

   #11795

   Purpose

   3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

   Depositor

   Luk Parijs

   Article

   Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

   Plasmid

   #212963

   Purpose

   Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

   Depositor

   Roland Rad

   Article

   Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

   Insert

   DR1_CasRx_GFP targeting spacer
   

   Use

   CRISPR and Lentiviral

   Tags

   TagRFP657

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAL-7xHis-TEV-hexa

   Plasmid

   #222868

   Purpose

   Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

   Depositor

   Nikolaus Loening

   Article

   Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

   Insert

   TEV protease
   

   Tags

   7xHis and MBP

   Expression

   Bacterial

   Mutation

   C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2060

   Plasmid

   #141899

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBMNb-cALFA

   Plasmid

   #159991

   Purpose

   For the protein expression with ALFA tag at C-terminus in mammalian cells

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   HRV3C protease cleavage site-ALFA tag-twin Strep …

   Expression

   Mammalian

   Mutation

   CMV promoter, WPRE

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. (133) pcDNA3.1-Nup62-Flag-EPEA

   Plasmid

   #160744

   Purpose

   Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   Nup62-Flag-EPEA
   

   Tags

   EPEA and Flag

   Expression

   Mammalian

   Promoter

   T7/CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_TetO_CD19-P2A-mCherry

   Plasmid

   #133803

   Purpose

   repressible expression of CD19 and mCherry in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   putative ECD of H. sapiens CD19 (CD19 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

   Plasmid

   #181745

   Purpose

   dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   human/zebrafish codon optimized SpCas9
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1X-Nup88

   Plasmid

   #87338

   Purpose

   To express human Nup88 in mammalian cells

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP88 (NUP88 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. p413 TEF NAPstarC

    Plasmid

    #232737

    Purpose

    Expresses control sensor NAPstarC in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstarC
    

    Expression

    Yeast

    Available Since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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