We narrowed to 27,832 results for: sta

1. pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

   Plasmid

   #92121

   Purpose

   CRISPR-Cas9 based efficient genome editing in S. aureus

   Depositor

   Sheng Yang

   Article

   Liu et al Acta Biochim Biophys Sin (Shanghai). 201

   Insert

   Cas9-Pxyltet-sgRNA-pj23119
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. c-myc promoter (Del 2)

   Plasmid

   #16602

   Depositor

   Bert Vogelstein

   Article

   He et al Science. 1998 Sep 4. 281(5382):1509-12.

   Insert

   c-myc promoter (Del 2) (MYC Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Del-2 (KpnI->PvuII). The KpnI site is at -1194…

   Available Since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

3. mScarlet-MYO10

   Plasmid

   #145179

   Purpose

   Expresses mScarlet-MYO10 in mammalian cells

   Depositor

   Johanna Ivaska

   Article

   Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

   Insert

   mScarlet-MYO10 (MYO10 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Syn-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

   Plasmid

   #222742

   Purpose

   CaST-IRES for neuron expression

   Depositor

   Christina Kim

   Article

   Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

   Insert

   CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. c-myc promoter (Del 3)

   Plasmid

   #16603

   Depositor

   Bert Vogelstein

   Article

   He et al Science. 1998 Sep 4. 281(5382):1509-12.

   Insert

   c-myc promoter (Del 3) (MYC Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Del-3 (AccI->PvuII). The AccI site is at -741 …

   Available Since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

6. Pveg

   Plasmid

   #55173

   Purpose

   Very strong constitutive promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   veg promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Promoter

   veg

   Available Since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEX-CMV-SP-STIM2(15-746)

   Plasmid

   #18868

   Depositor

   Tobias Meyer

   Article

   Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

   Insert

   STIM2 (STIM2 Human)
   

   Expression

   Mammalian

   Mutation

   Signal peptide(SP) 1-10 from STIM1 and residues 1…

   Available Since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-BsaIx2-DD-Cas9

   Plasmid

   #75380

   Purpose

   Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   DD-SpCas9
   

   Use

   CRISPR

   Tags

   FKBP12 L106P DD

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX303-GFP

   Plasmid

   #162032

   Purpose

   Tagged vector control

   Depositor

   David Bryant

   Article

   Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-TRAF6-wt

    Plasmid

    #21624

    Depositor

    John Kyriakis

    Article

    Zhong et al Mol Cell Biol. 2004 Oct . 24(20):9165-75

    Insert

    TNF receptor-associated factor 6 (Traf6 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCaRNA1&2

    Plasmid

    #86087

    Purpose

    Simultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cells

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    RNA1, RNA2
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCaRNA3

    Plasmid

    #86088

    Purpose

    Expression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cells

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    RNA3
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. mTagBFP2-TOMM20-N-10

    Plasmid

    #55328

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCAG-Bmpr2

    Plasmid

    #163403

    Purpose

    Expresses BMPR-2 in mammalican cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. mVenus-IQ6 WT

    Plasmid

    #198201

    Purpose

    FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    MYO5A IQ domain (Myo5a Mouse)
    

    Tags

    mVenus

    Expression

    Mammalian

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSupAR-MbPylRS(DiZPK)

    Plasmid

    #91705

    Purpose

    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest

    Depositor

    P. Chen

    Article

    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d

    Inserts

    pylS
    pylT
    

    Expression

    Bacterial

    Mutation

    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Flag-gp130dimer

    Plasmid

    #174394

    Purpose

    Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    IL6ST cytosolic domain with leucine zipper & C-C linker
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

    Plasmid

    #61594

    Purpose

    A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Insert

    hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX_TRE3G_DD::mCherry

    Plasmid

    #108679

    Purpose

    Tunable system for controlled gene expression and protein stability in mammalian cells

    Depositor

    Lucia Marucci

    Article

    Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

    Insert

    Destabilizing Domain (DD) mCherry fusion protein
    

    Use

    Lentiviral

    Tags

    DD::mCherry fusion protein

    Expression

    Mammalian

    Promoter

    Doxycycline inducible

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-N1-MITF-D

    Plasmid

    #38133

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-D (MITF Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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