We narrowed to 12,223 results for: EMB

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PDEST-pSP172BSSPE-pFOGc::RfA-ECFP

Plasmid

#186403

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter ECFP under control of the pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-pFOGc::RfA-venus

Plasmid

#186401

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-pFOGc::venus-RfA

Plasmid

#186400

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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KBB221

Plasmid

#185071

Purpose

SST::ACT2 "SA236"

Depositor

Ken Belanger

Article

Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

Insert

ACT2

Expression

Yeast

Mutation

ACT2-SA236

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KBB556

Plasmid

#185108

Purpose

1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with S158A, F159A, F160A

Depositor

Ken Belanger

Article

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Tags

GFP

Expression

Yeast

Mutation

1st 200 amino acids (N-term + transmembrane domai…

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KBB553

Plasmid

#185107

Purpose

1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFP with Y180L, Q182L

Depositor

Ken Belanger

Article

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Tags

GFP

Expression

Yeast

Mutation

1st 200 amino acids (N-term + transmembrane domai…

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-HA

Plasmid

#186410

Purpose

Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter HA tag under control of an AttB3/B5 recombined reg. sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-dsRNAres_H

Plasmid

#146502

Purpose

Insect Expression of DmDCP1

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1 (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-D34R-D87R-EGFP-6H

Plasmid

#187188

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

D34R, D87R

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-L32F-L33F-EGFP-6H

Plasmid

#187184

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

L32F, L33F

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-Y90A-V91A-EGFP-6H

Plasmid

#187185

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

Y90A, V91A

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-L32A-L33A-EGFP-6H

Plasmid

#187183

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

L32A, L33A

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-hs-AKT1-PH-EGFP-6H

Plasmid

#187157

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000142208 (AKT1 Human)

Tags

EGFP

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-D34K-D87K-EGFP-6H

Plasmid

#187187

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

D34K, D87K

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-miRFP670-eDHFR(69K6)

Plasmid

#178852

Purpose

Mammalian expression of miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-mNG-eDHFR(69K6)

Plasmid

#178853

Purpose

Mammalian expression of mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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CcCDP_p15A

Plasmid

#179272

Purpose

Expresses CcCDP in E. coli BL21(DE3)

Depositor

Bernd Nidetzky

Article

Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

Insert

cellodextrin phosphorylase

Use

Synthetic Biology

Tags

6xHIS

Expression

Bacterial

Promoter

T7 lacO

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCA528 CHMP1B 4-199

Plasmid

#115325

Purpose

Expresses residues 4-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-23

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Tags

His-SUMO

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_sgAgo2_Nterm

Plasmid

#170920

Purpose

Expresses spCas9 and sgRNA targeting the N-terminus of Ago2 for N-terminal tagging

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo2 (Ago2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA

Plasmid

#170767

Purpose

Methylase expression vector for 5RM assembly: expresses M.XbaI, M.EcoRI and M.SalI DNA methyltransferases in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Inserts

M.XbaI
M.EcoRI
M.SalI

Expression

Bacterial

Promoter

rhamnose

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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