We narrowed to 30,938 results for: TRO

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pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE

Plasmid

#111393

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
GCaMP6f

Use

AAV

Tags

6xHis, Myc, T7, and Xpress

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

Plasmid

#51247

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907 mutated to Ala

Promoter

EF1a

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3 HRPT2 227X

Plasmid

#11050

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 227X (CDC73 Human)

Tags

flag

Expression

Mammalian

Mutation

truncation mutant with a stop codon introduced at…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pMW41: pET-His-MBP-SUMO-R2Bm-SII

Plasmid

#209290

Purpose

Expresses the R2Bm ORF in E. coli

Depositor

Feng Zhang

Article

Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

Insert

R2

Tags

His, MBP, StrepII, and bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RasAR-NES

Plasmid

#205567

Purpose

Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

RasAR-NES (RAF1 Human, Synthetic)

Tags

Nuclear export signal (NES)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-ESRRA

Plasmid

#170683

Purpose

doxycycline-inducible overexpression of ESRRA

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

ESRRA (ESRRA Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-RKIP_RFP

Plasmid

#199215

Purpose

RKIP (PEBP1) expression plasmid with a RFP reporter on the backbone.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Inserts

RKIP (PEBP1 Human)
RFP

Expression

Mammalian

Promoter

CMV and PGK

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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IFNGR2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176939

Purpose

Mammalian expression vector encoding IFNGR2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

IFNGR2 (IFNGR2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT

Plasmid

#132667

Purpose

Expresses hypoxia-inducible Cas9 and TdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-ODD-T2A-TdT (DNTT Human, Streptococcus pyogenes)

Expression

Mammalian

Promoter

4xHRE_YB TATA

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763)

Plasmid

#39858

Depositor

Jonathon Pines

Article

Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

Insert

C11orf51 (ANAPC15 Human)

Tags

IRES2-mRuby

Expression

Mammalian

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-IP-EGFP-ULK1(1-828)

Plasmid

#38198

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 829-1051

Available Since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
pExT

Plasmid

#36889

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

T (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Hygro

Expression

Mammalian

Mutation

deleted nucleotides before nucleotide 275 and ins…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-hMcl-1(T163A)

Plasmid

#25376

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

hMCL1 (MCL1 Human)

Tags

V5 and His tag

Expression

Mammalian

Mutation

changed threonine 163 to alanine

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HIS HSPH1

Plasmid

#19534

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 105kDa/110kDa protein 1 (HSPH1 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA-p160(400-580)

Plasmid

#45

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Mutation

expresses only aa400-580

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
p156 pWZL-EGFP-SLPI

Plasmid

#11069

Depositor

Ramesh Shivdasani

Article

Schulze et al Blood. 2004 Dec 15. 104(13):3949-57.

Insert

SLPI (Slpi Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA-p160(200-400)

Plasmid

#44

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Mutation

Expresses only aa200-400

Available Since

July 21, 2005

Availability

Academic Institutions and Nonprofits only
Flag- MCL1-3A (S159, S162, T163 mutated to A)

Plasmid

#197458

Purpose

MCL-1 phosphodegron mutants

Depositor

Omar Khan

Article

Keyan et al Cancers (Basel). 2023 Jan 13;15(2):505.

Insert

MCL1 (MCL1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S159A, S161A, T163A

Promoter

CMV

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG CLTX-CAR

Plasmid

#157743

Purpose

knock CLTX-CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

chlorotoxin containing chimeric antigen receptor targeting glioblastoma

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-DYRK1A(K188R)-2A-mCherry

Plasmid

#177166

Purpose

Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Lysine at position 188 substituted with Arginine.

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)

Use

AAV

Tags

2A peptide and mCherry

Expression

Mammalian

Mutation

Changed Lysine 188 to Arginine

Promoter

CMV with beta globin intron

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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