We narrowed to 17,871 results for: SHE

1. p5E-dr-myl7-pro (JDW 1148)

   Plasmid

   #229836

   Purpose

   A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   cmlc2/myl7
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE)

   Plasmid

   #214880

   Purpose

   Lentiviral vector encoding RfxCas13d targeting CD46 guide

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   hU6-crCD46-EFS-PuroR-WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-Ef1a-fDIO-GCaMP6f

   Plasmid

   #128315

   Purpose

   Expresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

   Depositor

   Patricia Jensen

   Article

   Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

   Has Service

   AAV1

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   His Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV_APOBEC1-UdgX-NG-nCas9

   Plasmid

   #163533

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   APOBEC1-UdgX-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HiBiT-FZD7

   Plasmid

   #195845

   Purpose

   Expresses human FZD7 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD7 (FZD7 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK5 bovine GRK5

   Plasmid

   #14690

   Depositor

   Robert Lefkowitz

   Article

   Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

   Insert

   GRK5 (GRK5 Bovine)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pDEST-FRT/T0-Flag-BRCA1

   Plasmid

   #71117

   Purpose

   mammalian expression of Flag-BRCA1 siRNA resistant construct

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO

   Available since

   Nov. 12, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCMV_UdgX-EE-UdgX-nCas9-UdgX

   Plasmid

   #163566

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-nCas9-UdgX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CaMKIIa-eArch 3.0-EYFP

   Plasmid

   #35516

   Purpose

   AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   Arch3.0-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Enhanced trafficking signal and ER export signal …

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-FLAG-MTOR-I2500F

    Plasmid

    #69014

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-I2500F (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Isoleucine 2500 to Phenylalanine

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-ATF6-(S1P-)

    Plasmid

    #32956

    Purpose

    For visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S1Protease site mutation: R415A/R416A

    Promoter

    CMV

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSLQ7820 pHR (hU6-crPURI-EFS-PuroR-WPRE)

    Plasmid

    #214883

    Purpose

    Lentiviral vector encoding RfxCas13d targeting PURI guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crPURI-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSLQ7854 pHR (hU6-crGLY-EFS-PuroR-WPRE)

    Plasmid

    #214884

    Purpose

    Lentiviral vector encoding RfxCas13d targeting GLY guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crGLY-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pME-3xNLS-mTagBFP2-Cre (JDW 918)

    Plasmid

    #224517

    Purpose

    A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3xNLS-mTagBFP2-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCGN-ATF6 (1-373)m1

    Plasmid

    #27174

    Purpose

    For mammalian expression of the cytoplasmic domain of human ATF6 with mutations eliminating DNA binding and with an HA tag.

    Depositor

    Ron Prywes

    Article

    Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains only aa 1-373 Amino acids 315–317 are c…

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pTargetF-FRT

    Plasmid

    #113637

    Purpose

    Derivative of pTargetF with gRNA targeting FRT site

    Depositor

    Olivier Lichtarge

    Article

    Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

    Insert

    sgRNA-FRT
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pJ23119

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKDsgRNA-FRT

    Plasmid

    #113398

    Purpose

    expresses gRNA for Cas9 FRT targetting

    Depositor

    Jan Michiels

    Article

    Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

    Insert

    exo, beta, gam, sgRNA-FRT
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid

    #218862

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8m
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 5HT2C-RNA2-Flag

    Plasmid

    #79679

    Purpose

    serotonin receptor 2C isoform 2 with C-terminal Flag tag, non-edited isoform

    Depositor

    Stefan Stamm

    Article

    Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

    Insert

    serotonin receptor 2C, RNA2 (HTR2C Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pHD-SPARC2-S-LexA::p65

    Plasmid

    #133564

    Purpose

    Swap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    LexA::p65
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    20X UAS

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge